{"schema":"https://ver.cy/schemas/card/1.0.0","id":"vr.tr.mutagenesis","code":"thing-q149299","url":"https://ver.cy/models/thing/q149299/","name":"mutagenesis","alternateNames":["chemical mutagenesis","insertional mutagenesis","amino acid replacement","somatic hypermutation"],"kind":"thing","status":"research-draft","version":"0.2.0-wave.4","language":"en","classifiers":{"family":"Thing Registry","category":"Activities and processes","entryKind":"thing","plane":"ACT","domain":["ACT.PRC"],"industry":[],"navPath":"","tags":[],"facets":{}},"whatItIs":"The process by which the genetic information of an organism is changed, producing mutations, occurring naturally through replication errors and radiation or deliberately in research, including chemical mutagenesis with agents such as ethyl methanesulfonate, insertional mutagenesis by transposons or viral vectors, a known risk in some early gene therapy trials, site-directed mutagenesis to create specific amino acid replacements in proteins, and somatic hypermutation, the programmed mutation of antibody genes in B cells; mutagenesis research is subject to biosafety rules.","purpose":"Let an agent explain mutagenesis, relay mechanisms, research methods at a conceptual level and applications from genetics sources, describe the named forms, and distinguish mutagenesis from mutation as an outcome, carcinogenesis, genome editing and natural selection, without providing protocols for creating harmful organisms.","scope":{"in":[],"out":[],"boundaries":[]},"distinguishingFeatures":["Genetic change","Natural or induced","Research tool","Linked to cancer risk"],"structure":{"bundles":[{"id":"understand","name":"Understand","description":"What mutagenesis is.","layers":[{"id":"definition","name":"Definition","description":"Definition.","findings":[{"id":"definition-finding","name":"Definition","description":"Definition.","questions":[{"text":"What is mutagenesis, and how does it differ from mutation, carcinogenesis, genome editing and natural selection?","kind":"definition"},{"text":"Is the question conceptual, about safety testing or history, rather than protocols for harmful organisms, which are out of scope?","kind":"boundary"}]}]},{"id":"forms","name":"Forms","description":"Named forms.","findings":[{"id":"forms-finding","name":"Forms","description":"Forms.","questions":[{"text":"What are chemical and insertional mutagenesis, amino acid replacement and somatic hypermutation?","kind":"definition"},{"text":"Which entry fits the specific form?","kind":"action"}]}]}]},{"id":"science","name":"Science","description":"Mechanisms.","layers":[{"id":"mechanisms","name":"Mechanisms","description":"Mechanisms.","findings":[{"id":"mechanisms-finding","name":"Mechanisms","description":"Mechanisms.","questions":[{"text":"How do chemicals, radiation and replication errors cause mutations?","kind":"provenance"},{"text":"Which references are standard?","kind":"provenance"}]}]},{"id":"immunity","name":"Immunity","description":"Somatic hypermutation.","findings":[{"id":"immunity-finding","name":"Immunity","description":"Immunity.","questions":[{"text":"How does somatic hypermutation improve antibodies?","kind":"provenance"},{"text":"Which sources are cited?","kind":"provenance"}]}]}]},{"id":"applications","name":"Applications","description":"Applications.","layers":[{"id":"testing","name":"Testing","description":"Mutagenicity testing.","findings":[{"id":"testing-finding","name":"Testing","description":"Testing.","questions":[{"text":"How are chemicals screened for mutagenicity with the Ames test?","kind":"provenance"},{"text":"Which entry fits Ames test?","kind":"action"}]}]},{"id":"therapy","name":"Gene therapy","description":"Insertional risks.","findings":[{"id":"therapy-finding","name":"Gene therapy","description":"Gene therapy.","questions":[{"text":"What insertional mutagenesis risks were seen in early gene therapy trials, as reported?","kind":"provenance"},{"text":"Which entry fits gene therapy?","kind":"action"}]}]}]},{"id":"context","name":"Context","description":"Agriculture and history.","layers":[{"id":"breeding","name":"Breeding","description":"Mutation breeding.","findings":[{"id":"breeding-finding","name":"Breeding","description":"Breeding.","questions":[{"text":"How have induced mutations produced crop varieties?","kind":"provenance"},{"text":"Which entry fits mutation breeding?","kind":"action"}]}]},{"id":"history","name":"History","description":"History.","findings":[{"id":"history-finding","name":"History","description":"History.","questions":[{"text":"How did Muller s and Auerbach s work establish induced mutation?","kind":"provenance"},{"text":"Which entry fits Hermann Joseph Muller?","kind":"action"}]}]}]}]},"agentConduct":{"may":["explain mechanisms conceptually","relay applications","describe named forms","distinguish related processes"],"mustNot":[],"requiresHuman":[]},"ethics":{"considerations":["Let an agent explain mutagenesis, relay mechanisms, research methods at a conceptual level and applications from genetics sources, describe the named forms, and distinguish mutagenesis from mutation as an outcome, carcinogenesis, genome editing and natural selection, without providing protocols for creating harmful organisms.","Providing protocols for harmful organisms, which is out of scope"],"affectedParties":[]},"owners":{"steward":"","roles":[],"masterSystems":[]},"relations":[{"target":"Q6804646","type":"parent","note":"registry parent class"},{"target":"biological mechanism","type":"related","note":"in registry terms"},{"target":"mutation","type":"related"},{"target":"site-directed mutagenesis","type":"related"},{"target":"genome editing","type":"related"}],"interaction":{"identity":{"applicability":"required","items":["Vercy registry: vr.tr.mutagenesis","Wikidata: Q149299 (https://www.wikidata.org/wiki/Q149299)"]},"properties":{"applicability":"not-applicable","items":["Muller X-ray mutagenesis: 1927 year - Nobel 1946","site-directed mutagenesis Nobel: 1993 year - Michael Smith","Ames test: 1970s note - mutagenicity screening"]},"recognition":{"applicability":"optional","items":["Process producing genetic mutations","Chemical mutagenesis, insertional mutagenesis, amino acid replacement, somatic hypermutation","Mutations are outcomes; carcinogenesis is cancer development; genome editing is targeted; selection acts on variation","Not a visible object; detected through sequencing and phenotypes."]},"capabilities":{"applicability":"required","items":["explain mechanisms conceptually","relay applications","describe named forms","distinguish related processes"]},"hazards":{"applicability":"required","items":["Providing protocols for harmful organisms, which is out of scope","Confusing mutagens and carcinogens","Oversimplifying mutation effects"]},"interfaces":{"applicability":"required","items":["Biosafety and GMO regulations","OECD mutagenicity test guidelines such as the Ames test"]},"context":{"applicability":"required","items":["Not applicable; a biological process and research method.","spontaneous mutagenesis","chemical and radiation mutagenesis","insertional mutagenesis","site-directed mutagenesis","somatic hypermutation","mutation breeding in crops"]}},"sources":[],"openQuestions":["Should somatic hypermutation be a separate entry?","How should genetics sources be linked?","Should amino acid replacement be moved to protein engineering?","Which entry fits the specific form?","Which entry fits Ames test?","Which entry fits gene therapy?"],"resources":{"spec":"/models/things/publications/thing-q149299/spec.json"},"provenance":{"origin":"thing registry research (pass 2)","builtFrom":["models/things/publications/thing-q149299/spec.json"],"providers":["Claude"],"researchStatus":"unreviewed","generatedAt":"2026-09-13T20:39:27Z","builder":"tools/build_cards.py@1.0.0"},"completeness":{"sections":{"classifiers":"filled","whatItIs":"filled","purpose":"filled","distinguishingFeatures":"filled","structure":"filled","agentConduct":"derived","ethics":"derived","owners":"missing","relations":"filled","interaction.identity":"filled","interaction.properties":"not-applicable","interaction.recognition":"filled","interaction.capabilities":"filled","interaction.hazards":"filled","interaction.interfaces":"filled","interaction.context":"filled","sources":"missing"},"notes":{"agentConduct":"Affordances listed as what may be done; prohibitions collected from the text.","interaction.properties":"Plane ACT: no invented physical properties.","sources":"Written from model knowledge without web access; claims are unverified."},"score":0.812}}