{"schema":"https://ver.cy/schemas/card/1.0.0","id":"vr.tr.faicar","code":"thing-q2817110","url":"https://ver.cy/models/thing/q2817110/","name":"faicar","alternateNames":[],"kind":"thing","status":"published","version":"0.1.0-research.1","language":"en","classifiers":{"family":"Thing Registry","category":"Physical world and living systems","entryKind":"thing","plane":"PHY","domain":["PHY.MAT"],"industry":[],"navPath":"","tags":[],"facets":{}},"whatItIs":"FAICAR is 5-formamidoimidazole-4-carboxamide ribonucleotide, a formylated nucleotide intermediate in de novo purine biosynthesis that undergoes ring closure to form inosine monophosphate.","purpose":"Enable an agent to identify FAICAR, assess the identity and condition of a FAICAR-containing sample, and judge its suitability for studying purine biosynthesis.","scope":{"in":["FAICAR molecular identity, ribose stereochemistry and 5'-phosphate attachment","Protonation, counterions, hydration and their effects on reported composition","FAICAR concentration, purity and distinction from related pathway metabolites","Formation from AICAR and conversion to inosine monophosphate","Sample preservation, assay compatibility and handling evidence"],"out":["AICAR, IMP and other purine-pathway metabolites as independently owned substances","ATIC, PurH and PurP enzyme structures and complete catalytic models","The complete purine biosynthesis pathway and its regulation","Organism-wide metabolic states or clinical interpretations","Commercial reagent formulations and laboratory instruments as independent things"],"boundaries":[]},"distinguishingFeatures":["The proposed identity contains a formamido substituent where AICAR has an amino substituent; identification must resolve this chemical difference.","The proposed identity includes a ribose-linked 5'-phosphate, distinguishing it from corresponding unphosphorylated compounds.","FAICAR retains the substituted imidazole structure preceding ring closure to IMP; an IMP signal does not establish FAICAR identity.","The reference neutral-acid formula is C10H15N4O9P and the PubChem CID is 166760; formula agreement alone does not establish connectivity or stereochemistry. [PubChem FAICAR](https://pubchem.ncbi.nlm.nih.gov/compound/Faicar)"],"structure":{"bundles":[{"id":"faicar-identity","name":"FAICAR identity","description":"Establish what chemical entity the registry name denotes and which material forms represent it.","layers":[{"id":"name-and-structure","name":"Name and structure","description":"Connect the registered name to a chemically explicit reference.","findings":[{"id":"reference-identity","name":"Reference identity","description":"PubChem identifies FAICAR as a formamidoimidazole carboxamide ribonucleotide with CID 166760; linking that identity to this registry entry remains a provenance task. [PubChem FAICAR](https://pubchem.ncbi.nlm.nih.gov/compound/Faicar)","questions":[{"text":"Which originating registry record or external identifier confirms that vr.tr.faicar denotes this FAICAR structure?","id":"reference-identity-q01","kind":"provenance"},{"text":"Does the assigned structure preserve the formamido group, ribose stereochemistry and 5'-phosphate attachment?","id":"reference-identity-q02","kind":"definition"}]}]},{"id":"forms-and-neighbours","name":"Forms and neighbours","description":"Separate representations of FAICAR from chemically different metabolites.","findings":[{"id":"form-boundary","name":"Chemical form boundary","description":"The model must distinguish protonation and salt descriptions from changes in connectivity, phosphorylation or stereochemistry.","questions":[{"text":"Which protonation state, counterions and hydration state describe the actual FAICAR material?","id":"form-boundary-q01","kind":"definition"},{"text":"Does a candidate synonym denote FAICAR itself, an unphosphorylated analogue, AICAR or another substance?","id":"form-boundary-q02","kind":"boundary"}]}]}]},{"id":"purine-intermediate-role","name":"Purine intermediate role","description":"Represent FAICAR's immediate formation and consumption reactions.","layers":[{"id":"faicar-formation","name":"FAICAR formation","description":"Record the reaction that supplies FAICAR in the system under examination.","findings":[{"id":"aicar-formylation","name":"AICAR formylation","description":"Human AICAR transformylase transfers a formyl group from 10-formyltetrahydrofolate to AICAR to form FAICAR. [Primary biochemical study](https://www.sciencedirect.com/science/article/pii/S002192581932109X)","questions":[{"text":"Which organism, enzyme and formyl donor account for FAICAR formation in this sample?","id":"aicar-formylation-q01","kind":"provenance"},{"text":"What measurement distinguishes newly formed FAICAR from residual AICAR and other reaction components?","id":"aicar-formylation-q02","kind":"measurement"}]}]},{"id":"faicar-consumption","name":"FAICAR consumption","description":"Connect FAICAR to its immediate IMP-forming reaction without absorbing the enzyme model.","findings":[{"id":"imp-ring-closure","name":"IMP ring closure","description":"IMP cyclohydrolase catalyses the reversible conversion of FAICAR to IMP and water. [Reactome reaction](https://reactome.org/content/detail/R-HSA-73797)","questions":[{"text":"Which IMP cyclohydrolase and experimental conditions apply to the proposed FAICAR conversion?","id":"imp-ring-closure-q01","kind":"provenance"},{"text":"Which controls and measurements establish FAICAR consumption together with IMP formation?","id":"imp-ring-closure-q02","kind":"measurement"}]}]}]},{"id":"faicar-analytical-confidence","name":"FAICAR analytical confidence","description":"Determine whether a reported signal and quantity genuinely represent FAICAR.","layers":[{"id":"identity-evidence","name":"Identity evidence","description":"Evaluate measurements that resolve FAICAR from plausible alternatives.","findings":[{"id":"discriminating-identification","name":"Discriminating identification","description":"Identity evidence should document a method capable of distinguishing FAICAR from AICAR, IMP and relevant isomeric or dephosphorylated candidates.","questions":[{"text":"Which reference standard, chromatographic, mass-spectral or structural evidence supports the FAICAR assignment?","id":"discriminating-identification-q01","kind":"measurement"},{"text":"Which plausible alternative compounds remain unresolved by the identification method?","id":"discriminating-identification-q02","kind":"boundary"}]}]},{"id":"amount-and-impurities","name":"Amount and impurities","description":"Separate quantified FAICAR from the overall preparation.","findings":[{"id":"usable-faicar-amount","name":"Usable FAICAR amount","description":"Quantification must distinguish FAICAR content from total solids, detector area and nominal preparation concentration.","questions":[{"text":"What calibration, recovery and uncertainty support the reported FAICAR concentration?","id":"usable-faicar-amount-q01","kind":"measurement"},{"text":"How much AICAR, IMP or other relevant impurity is present, and does it interfere with the intended assay?","id":"usable-faicar-amount-q02","kind":"measurement"}]}]}]},{"id":"faicar-sample-fitness","name":"FAICAR sample fitness","description":"Assess whether a particular preparation remains suitable for its intended use.","layers":[{"id":"preservation-and-integrity","name":"Preservation and integrity","description":"Tie storage and preparation conditions to measured retention of FAICAR.","findings":[{"id":"condition-dependent-integrity","name":"Condition-dependent integrity","description":"Acceptable storage and working conditions require evidence for FAICAR under the relevant solvent, pH, temperature and duration; this draft assigns no universal stability limit.","questions":[{"text":"What fraction of FAICAR remains after the actual storage and handling history?","id":"condition-dependent-integrity-q01","kind":"measurement"},{"text":"What evidence establishes the maximum working time and conditions before reanalysis is required?","id":"condition-dependent-integrity-q02","kind":"action"}]}]},{"id":"assay-and-handling-decisions","name":"Assay and handling decisions","description":"Link preparation-specific evidence to a concrete use decision.","findings":[{"id":"preparation-release","name":"Preparation release","description":"Use decisions should consider FAICAR identity and concentration alongside counterions, residual synthesis components, buffer compatibility and documented handling requirements.","questions":[{"text":"Do the preparation's composition and analytical results meet the stated requirements of the intended FAICAR assay?","id":"preparation-release-q01","kind":"action"},{"text":"Which safety documentation applies to this exact FAICAR form or mixture, and what handling requirements does it establish?","id":"preparation-release-q02","kind":"provenance"}]}]}]},{"id":"evidence-and-external-alignment","name":"Evidence and external alignment","description":"What the world already says about this thing, gathered so the model can be checked against it.","layers":[{"id":"reported-evidence","name":"Reported evidence","description":"Findings from the breadth pass, kept separate from the structural claims.","findings":[{"id":"evidence-confidence-notes","name":"Check these first","description":"Recalled without web access and unsourced; every item is a lead to verify.","questions":[{"text":"Which of these check these first hold for the sense of faicar this model covers, and on what evidence?","id":"evidence-confidence-notes-q01","kind":"provenance"}]},{"id":"evidence-identifiers","name":"Identifiers and schemes","description":"Recalled without web access and unsourced; every item is a lead to verify.","questions":[{"text":"Which of these identifiers and schemes hold for the sense of faicar this model covers, and on what evidence?","id":"evidence-identifiers-q01","kind":"provenance"}]},{"id":"evidence-real-world-use","name":"Real-world use","description":"Recalled without web access and unsourced; every item is a lead to verify.","questions":[{"text":"Which of these real-world use hold for the sense of faicar this model covers, and on what evidence?","id":"evidence-real-world-use-q01","kind":"provenance"}]},{"id":"evidence-neighbours","name":"Neighbouring kinds and how to tell them apart","description":"Recalled without web access and unsourced; every item is a lead to verify.","questions":[{"text":"Which of these neighbouring kinds and how to tell them apart hold for the sense of faicar this model covers, and on what evidence?","id":"evidence-neighbours-q01","kind":"provenance"}]}]}]}]},"agentConduct":{"may":["Identify FAICAR as an intermediate of purine synthesis and report it with its source and evidence.","Describe its role in the pathway and the enzyme step that converts it, citing literature."],"mustNot":["Report a measurement of FAICAR in a sample without stating the method and its limits.","Present it as a supplement or therapy for people.","Confuse it with related nucleotides such as AICAR when stating results.","Infer a person's metabolic disorder from FAICAR data without clinical review."],"requiresHuman":["Interpreting metabolite data that bears on a patient's diagnosis.","Publishing findings that depend on an unverified FAICAR standard."]},"ethics":{"considerations":["Metabolic findings can lead to diagnoses that affect patients and families.","Errors in research reagents can waste effort and mislead later studies."],"affectedParties":["Patients with metabolic disorders","Researchers","Laboratory staff"]},"owners":{"steward":"The laboratory that holds the sample or reference material.","roles":[],"masterSystems":[]},"relations":[{"target":"Q84737692","type":"parent","note":"registry parent class"}],"interaction":{"identity":{"applicability":"required","items":["Vercy registry: vr.tr.faicar","Wikidata: Q2817110 (https://www.wikidata.org/wiki/Q2817110)"]},"properties":{"applicability":"required","items":["Chemical identity assignment: FAICAR reference match; tentative match; unresolved","Molecular form: Neutral-acid representation; specified ionic form; specified salt or hydrate; unknown","FAICAR concentration: µmol/L or mmol/L, with method, uncertainty and sampling time","FAICAR purity: Percentage with stated basis and method; separately quantified AICAR and IMP where measured","Solution conditions: Measured pH, temperature in °C, solvent, buffer and ionic composition","Biochemical role: Product of a documented AICAR formylation reaction; substrate of a documented IMP-forming reaction","Preservation history: Preparation time, storage temperature, elapsed storage, freeze-thaw count and latest integrity measurement"]},"recognition":{"applicability":"required","items":["Not identifiable by appearance; in practice a solution or small solid sample in a labelled vial.","Identity is settled by mass spectrometry or chromatography against a reference."]},"capabilities":{"applicability":"required","items":["Resolve a FAICAR label against a chemical structure and authoritative identifier.","Compare sample measurements with evidence distinguishing FAICAR from AICAR and IMP.","Calculate preparation amounts using the documented chemical form and measured purity.","Assess whether a sample is suitable as an IMP cyclohydrolase substrate under specified assay conditions.","Track FAICAR production or consumption while preserving organism, enzyme and sampling context.","Request reanalysis or withhold use when identity, preservation or handling evidence is insufficient."]},"hazards":{"applicability":"required","items":["Degradation or misidentification of the reagent gives false experimental results.","Confusion with similarly named nucleotides in reports."]},"interfaces":{"applicability":"required","items":["The Globally Harmonized System of Classification and Labelling of Chemicals, issued by the United Nations, provides the framework for evaluating hazards and communicating any applicable classifications for supplied FAICAR materials."]},"context":{"applicability":"required","items":["FAICAR is encountered in biochemical research on the final stages of de novo purine biosynthesis.","Researchers study it in enzyme assays and metabolic analyses to investigate its conversion to inosine monophosphate.","Its role as a pathway intermediate makes sample identity and stability relevant to interpreting experimental results."]}},"sources":[{"title":"Wikidata item Q2817110: faicar","url":"https://www.wikidata.org/wiki/Q2817110","note":"identity and sense of the item"},{"title":"Wikipedia: 5-Formamidoimidazole-4-carboxamide ribotide","url":"https://en.wikipedia.org/wiki/5-Formamidoimidazole-4-carboxamide_ribotide","note":"general description of the item"}],"openQuestions":["Does the originating registry identifier confirm that faicar denotes 5-formamidoimidazole-4-carboxamide ribonucleotide?","Which CAS, ChEBI and EC identifiers, if any, apply to the intended parent structure and its separately identified salts?","Which validated analytical methods and reference materials best distinguish and quantify FAICAR alongside AICAR and IMP?","What experimental evidence establishes FAICAR solubility, acid-base behaviour and stability under relevant preparation, storage and assay conditions?","Which preparation-specific safety classifications and handling requirements are supported by authoritative documentation?"],"resources":{"spec":"/models/things/publications/thing-q2817110/spec.json"},"provenance":{"origin":"thing registry research (pass 2)","builtFrom":["models/things/publications/thing-q2817110/spec.json"],"providers":["Codex"],"researchStatus":"unreviewed","generatedAt":"2026-09-12T09:37:13Z","builder":"tools/build_cards.py@1.0.0"},"completeness":{"sections":{"classifiers":"filled","whatItIs":"filled","purpose":"filled","distinguishingFeatures":"filled","structure":"filled","agentConduct":"filled","ethics":"filled","owners":"filled","relations":"filled","interaction.identity":"filled","interaction.properties":"filled","interaction.recognition":"filled","interaction.capabilities":"filled","interaction.hazards":"filled","interaction.interfaces":"filled","interaction.context":"filled","sources":"filled"},"notes":{"_gate":"Published through the thing publication gate 1.0 on 2026-10-07T10:10:29+00:00: completeness 1.00; sense: Wikidata description; 2 live sources; review by codex: pass"},"score":1.0}}