{"schema":"https://ver.cy/schemas/card/1.0.0","id":"vr.tr.digitonin","code":"thing-q410968","url":"https://ver.cy/models/thing/q410968/","name":"digitonin","alternateNames":[],"kind":"thing","status":"published","version":"0.1.0-research.1","language":"en","classifiers":{"family":"Thing Registry","category":"Physical world and living systems","entryKind":"thing","plane":"PHY","domain":["PHY.MAT"],"industry":[],"navPath":"","tags":[],"facets":{}},"whatItIs":"Digitonin is a steroidal saponin glycoside found in Digitalis plants that complexes with cholesterol and is used as a nonionic detergent to permeabilize or solubilize biological membranes.","purpose":"Enable an agent to identify digitonin, assess a particular preparation, and judge its suitability for cholesterol interaction, membrane permeabilization, or membrane-component solubilization.","scope":{"in":["Digitonin molecular identity and distinction from related Digitalis glycosides","Lot composition, purity, water content, and analytical evidence","Solid material, stock solutions, solubility, and aggregation under recorded conditions","Cholesterol interaction and experimentally established membrane effects","Storage, exposure controls, and disposition of digitonin preparations"],"out":["Digitalis plants and their cultivation","Digitogenin, digitoxin, and digoxin as independently modeled substances","Complete cell-permeabilization and organelle-isolation protocols","Biological specimens and their underlying membrane composition","Commercial reagent kits, packaging, and purchasing workflows","Therapeutic claims or clinical treatment regimens"],"boundaries":[]},"distinguishingFeatures":["Match the molecular structure, not merely a Digitalis-associated name: digitonin is a glycoside with digitogenin attached to five sugars. [PubChem](https://pubchem.ncbi.nlm.nih.gov/compound/Digitonin)","Check the identity against formula C56H92O29 and CAS 11024-24-1; the name alone does not establish lot purity. [PubChem](https://pubchem.ncbi.nlm.nih.gov/compound/Digitonin)","Distinguish intact digitonin from its aglycone digitogenin through evidence for the attached sugar portion. [PubChem](https://pubchem.ncbi.nlm.nih.gov/compound/Digitonin)","Distinguish its cholesterol-interacting, nonionic detergent role from an unspecified detergent designation; membrane activity alone is insufficient to establish chemical identity. [Sigma-Aldrich](https://www.sigmaaldrich.com/US/en/product/sigma/d141)"],"structure":{"bundles":[{"id":"digitonin-identity","name":"Digitonin identity","description":"Establish which molecular entity the registered name denotes.","layers":[{"id":"molecular-recognition","name":"Molecular recognition","description":"Recognize digitonin through structure and concordant identifiers.","findings":[{"id":"intact-glycoside-identity","name":"Intact glycoside identity","description":"Require evidence that identifies the intact digitonin glycoside rather than only its origin or aglycone.","questions":[{"text":"Which structural reference defines digitonin's stereochemistry and sugar connectivity?","id":"intact-glycoside-identity-q01","kind":"definition"},{"text":"Which analytical observations distinguish intact digitonin from digitogenin and related glycosides?","id":"intact-glycoside-identity-q02","kind":"measurement"}]}]},{"id":"registry-and-neighbour-boundaries","name":"Registry and neighbour boundaries","description":"Keep identifiers and adjacent chemical entities distinct.","findings":[{"id":"identifier-concordance","name":"Identifier concordance","description":"Reconcile registry, supplier, and chemical database records without treating every database number as a compound identifier.","questions":[{"text":"Do the CAS, EC, structure, and compound database record refer to the same digitonin entity?","id":"identifier-concordance-q01","kind":"provenance"},{"text":"Does a cited identifier denote digitonin itself, a supplier substance record, or a formulated solution?","id":"identifier-concordance-q02","kind":"boundary"}]}]}]},{"id":"digitonin-preparation-quality","name":"Digitonin preparation quality","description":"Describe the composition and performance evidence of an actual preparation.","layers":[{"id":"composition-and-assay","name":"Composition and assay","description":"Establish what fraction of the material is digitonin and how that was measured.","findings":[{"id":"purity-basis","name":"Purity basis","description":"Interpret assay values alongside water, residual solvents, and related components.","questions":[{"text":"Is the reported purity a mass fraction, chromatographic area percentage, or another assay result?","id":"purity-basis-q01","kind":"measurement"},{"text":"Which related saponins, degradation products, water, or residual solvents are quantified?","id":"purity-basis-q02","kind":"measurement"}]}]},{"id":"lot-fitness","name":"Lot fitness","description":"Connect traceable preparation history to a defined digitonin use.","findings":[{"id":"functional-lot-acceptance","name":"Functional lot acceptance","description":"Separate chemical identity acceptance from suitability for a particular membrane or cholesterol assay.","questions":[{"text":"Which certificate and preparation history support the identity and grade of this lot?","id":"functional-lot-acceptance-q01","kind":"provenance"},{"text":"What reference assay and acceptance threshold establish this lot's fitness for the intended digitonin application?","id":"functional-lot-acceptance-q02","kind":"action"}]}]}]},{"id":"digitonin-physical-state","name":"Digitonin physical state","description":"Track dissolution, aggregation, and physical changes that affect usable digitonin.","layers":[{"id":"dissolution-and-aggregation","name":"Dissolution and aggregation","description":"Describe concentration and solution behavior under explicit conditions.","findings":[{"id":"available-solution-concentration","name":"Available solution concentration","description":"Distinguish nominal loading, dissolved material, aggregates, and visible precipitate.","questions":[{"text":"What solvent, buffer, temperature, and equilibration conditions support the stated dissolved concentration?","id":"available-solution-concentration-q01","kind":"measurement"},{"text":"What evidence distinguishes undissolved digitonin from aggregation or precipitation after dilution?","id":"available-solution-concentration-q02","kind":"measurement"}]}]},{"id":"thermal-and-storage-behavior","name":"Thermal and storage behavior","description":"Assess whether preparation and storage preserve usable digitonin.","findings":[{"id":"state-change-versus-degradation","name":"State change versus degradation","description":"Record physical and chemical evidence separately when a preparation changes during heating or storage.","questions":[{"text":"Which reported thermal values distinguish melting from decomposition for the characterized material?","id":"state-change-versus-degradation-q01","kind":"measurement"},{"text":"Which storage duration, temperature, and solvent conditions preserve the stock's concentration and assay performance?","id":"state-change-versus-degradation-q02","kind":"action"}]}]}]},{"id":"cholesterol-dependent-function","name":"Cholesterol-dependent function","description":"Represent cholesterol interaction and its consequences for membrane experiments.","layers":[{"id":"sterol-interaction","name":"Sterol interaction","description":"Characterize the chemical interaction separately from whole-cell outcomes.","findings":[{"id":"cholesterol-complexation","name":"Cholesterol complexation","description":"Digitonin is used as a cholesterol-precipitating reagent; the model must retain the conditions and analytical endpoint of that use. [PubChem](https://pubchem.ncbi.nlm.nih.gov/compound/Digitonin)","questions":[{"text":"Under which solvent, concentration, and temperature conditions is cholesterol complexation or precipitation demonstrated?","id":"cholesterol-complexation-q01","kind":"measurement"},{"text":"Which other sterols or sample components interfere with the intended cholesterol measurement?","id":"cholesterol-complexation-q02","kind":"boundary"}]}]},{"id":"membrane-selectivity","name":"Membrane selectivity","description":"Describe the experimentally supported window between useful access and excessive membrane disruption.","findings":[{"id":"permeabilization-window","name":"Permeabilization window","description":"Treat selective permeabilization as an observed relationship among the preparation, membrane system, and exposure conditions.","questions":[{"text":"At what digitonin concentration, exposure time, temperature, and specimen loading is the required permeability achieved?","id":"permeabilization-window-q01","kind":"measurement"},{"text":"Which paired measurements establish target-membrane access and retention of the required organelle integrity?","id":"permeabilization-window-q02","kind":"measurement"},{"text":"What observed endpoint requires reducing exposure or rejecting the preparation for this use?","id":"permeabilization-window-q03","kind":"action"}]}]},{"id":"solubilization-and-assay-effects","name":"Solubilization and assay effects","description":"Separate membrane-component extraction from preservation of downstream function.","findings":[{"id":"extraction-fitness","name":"Extraction fitness","description":"Assess whether digitonin exposure yields material compatible with the intended biochemical measurement.","questions":[{"text":"Which measurements establish extraction yield and preservation of the target protein or complex's required activity?","id":"extraction-fitness-q01","kind":"measurement"},{"text":"Does residual digitonin alter the downstream readout, and what evidence establishes an acceptable residual level?","id":"extraction-fitness-q02","kind":"action"}]}]}]},{"id":"digitonin-handling-constraints","name":"Digitonin handling constraints","description":"Attach evidence-based handling decisions to digitonin powder and its preparations.","layers":[{"id":"hazard-evidence","name":"Hazard evidence","description":"Resolve applicable classifications and exposure information for the material in hand.","findings":[{"id":"preparation-specific-hazards","name":"Preparation-specific hazards","description":"Keep digitonin hazard evidence distinct from solvent hazards and undocumented assumptions about toxicity.","questions":[{"text":"Which current supplier SDS and jurisdiction establish the hazard classification of this digitonin preparation?","id":"preparation-specific-hazards-q01","kind":"provenance"},{"text":"Are digitonin-specific exposure limits established, absent, or simply not yet checked?","id":"preparation-specific-hazards-q02","kind":"provenance"}]}]},{"id":"handling-and-disposition","name":"Handling and disposition","description":"Translate the preparation's state and documentation into practical controls.","findings":[{"id":"powder-stock-and-waste-controls","name":"Powder, stock, and waste controls","description":"Select controls for weighing, solution preparation, storage, and contaminated waste using the actual preparation and use context.","questions":[{"text":"What documented controls apply when weighing digitonin powder or preparing stocks in the selected solvent?","id":"powder-stock-and-waste-controls-q01","kind":"action"},{"text":"How do digitonin, solvent, and biological contaminants jointly determine the applicable waste route?","id":"powder-stock-and-waste-controls-q02","kind":"action"}]}]}]},{"id":"evidence-and-external-alignment","name":"Evidence and external alignment","description":"What the world already says about this thing, gathered so the model can be checked against it.","layers":[{"id":"reported-evidence","name":"Reported evidence","description":"Findings from the breadth pass, kept separate from the structural claims.","findings":[{"id":"evidence-confidence-notes","name":"Check these first","description":"Recalled without web access and unsourced; every item is a lead to verify.","questions":[{"text":"Which of these check these first hold for the sense of digitonin this model covers, and on what evidence?","id":"evidence-confidence-notes-q01","kind":"provenance"}]},{"id":"evidence-identifiers","name":"Identifiers and schemes","description":"Recalled without web access and unsourced; every item is a lead to verify.","questions":[{"text":"Which of these identifiers and schemes hold for the sense of digitonin this model covers, and on what evidence?","id":"evidence-identifiers-q01","kind":"provenance"}]},{"id":"evidence-real-world-use","name":"Real-world use","description":"Recalled without web access and unsourced; every item is a lead to verify.","questions":[{"text":"Which of these real-world use hold for the sense of digitonin this model covers, and on what evidence?","id":"evidence-real-world-use-q01","kind":"provenance"}]},{"id":"evidence-measurements","name":"Typical measurements","description":"Recalled without web access and unsourced; every item is a lead to verify.","questions":[{"text":"Which of these typical measurements hold for the sense of digitonin this model covers, and on what evidence?","id":"evidence-measurements-q01","kind":"provenance"}]},{"id":"evidence-failure-modes-and-hazards","name":"Failure modes and hazards","description":"Recalled without web access and unsourced; every item is a lead to verify.","questions":[{"text":"Which of these failure modes and hazards hold for the sense of digitonin this model covers, and on what evidence?","id":"evidence-failure-modes-and-hazards-q01","kind":"provenance"}]},{"id":"evidence-neighbours","name":"Neighbouring kinds and how to tell them apart","description":"Recalled without web access and unsourced; every item is a lead to verify.","questions":[{"text":"Which of these neighbouring kinds and how to tell them apart hold for the sense of digitonin this model covers, and on what evidence?","id":"evidence-neighbours-q01","kind":"provenance"}]}]}]}]},"agentConduct":{"may":["Identify digitonin as a saponin detergent used to permeabilize membranes, citing sources.","Report grade, storage and safety data of a held stock."],"mustNot":["Assist anyone in using it to harm people or animals.","Recommend it or foxglove material as a remedy.","Handle it without regard to its safety data.","Dispose of it in drains or general waste."],"requiresHuman":["Ordering, transferring or disposing of stock.","Using it in studies involving animals."]},"ethics":{"considerations":["It is toxic and comes from a poisonous plant, so careless handling harms workers.","Foxglove poisoning happens when plants are mistaken for edible ones."],"affectedParties":["Laboratory workers","People and animals near foxglove","Waste handlers"]},"owners":{"steward":"The laboratory that holds the stock.","roles":[],"masterSystems":["Laboratory chemical inventories"]},"relations":[{"target":"Q77806815","type":"parent","note":"registry parent class"}],"interaction":{"identity":{"applicability":"required","items":["Vercy registry: vr.tr.digitonin","Wikidata: Q410968 (https://www.wikidata.org/wiki/Q410968)"]},"properties":{"applicability":"required","items":["Chemical identity: Digitonin; CAS 11024-24-1; PubChem CID 6474107; EC 234-255-6","Formula and molar mass: C56H92O29; approximately 1229.3 g/mol","Assay purity and composition: % with analytical method and basis; related-component profile; water content","Preparation state: Solid, dissolved, dispersed, or precipitated; solvent and temperature recorded","Solution concentration: mg/mL or mmol/L; distinguish nominal from analytically measured concentration","Solubility and aggregation behavior: Solubility in mg/mL; aggregation threshold in mmol/L if established; solvent, buffer, temperature, and method","Thermal behavior: Melting or decomposition temperature in °C with method; boiling point only if established","Membrane response: Preparation linked to specimen, exposure conditions, permeability endpoint, and organelle-integrity endpoint","Hazard and handling status: Applicable SDS classifications, jurisdiction, revision date, exposure limits if established, and required controls"]},"recognition":{"applicability":"required","items":["A white powder supplied in labelled containers; not identifiable by sight.","Its source plant, foxglove, has tall spikes of tubular purple or white flowers."]},"capabilities":{"applicability":"required","items":["Authenticate a preparation against digitonin identity references and lot-specific analytical evidence.","Compare lots for a specified membrane assay using both purity and functional acceptance criteria.","Evaluate a proposed stock solution against documented dissolution and stability evidence.","Assess whether measured exposure conditions support selective permeabilization or broader solubilization.","Evaluate digitonin for cholesterol precipitation or complexation using a defined analytical endpoint.","Determine handling, storage, and disposal requirements from applicable preparation-specific documentation."]},"hazards":{"applicability":"required","items":["Toxic exposure through ingestion or inhalation of dust.","Variable purity giving inconsistent results."]},"interfaces":{"applicability":"required","items":["Globally Harmonized System of Classification and Labelling of Chemicals (GHS), United Nations."]},"context":{"applicability":"required","items":["Digitonin is encountered in cell biology and biochemistry laboratories as a reagent for working with cholesterol-containing membranes.","Researchers use it to permeabilize cells or extract membrane components when the preparation must retain features needed for subsequent analysis."]}},"sources":[{"title":"Wikidata item Q410968: digitonin","url":"https://www.wikidata.org/wiki/Q410968","note":"identity and sense of the item"},{"title":"Wikipedia: Digitonin","url":"https://en.wikipedia.org/wiki/Digitonin","note":"general description of the item"}],"openQuestions":["Which structural reference resolves legacy or conflicting digitonin database identifiers without conflating compound and supplier substance records?","How much do related saponins, water content, and preparation method vary across available grades, and which differences change functional potency?","What measured solubility, aggregation, thermal behavior, and stock stability apply under the intended working conditions?","Which specimen-specific exposure window achieves the required permeability while preserving the organelles or protein complexes relevant to the task?","What current jurisdiction-specific hazard classification, exposure-limit status, and disposal requirements apply to the selected preparation?"],"resources":{"spec":"/models/things/publications/thing-q410968/spec.json"},"provenance":{"origin":"thing registry research (pass 2)","builtFrom":["models/things/publications/thing-q410968/spec.json"],"providers":["Codex"],"researchStatus":"unreviewed","generatedAt":"2026-09-12T09:10:38Z","builder":"tools/build_cards.py@1.0.0"},"completeness":{"sections":{"classifiers":"filled","whatItIs":"filled","purpose":"filled","distinguishingFeatures":"filled","structure":"filled","agentConduct":"filled","ethics":"filled","owners":"filled","relations":"filled","interaction.identity":"filled","interaction.properties":"filled","interaction.recognition":"filled","interaction.capabilities":"filled","interaction.hazards":"filled","interaction.interfaces":"filled","interaction.context":"filled","sources":"filled"},"notes":{"_gate":"Published through the thing publication gate 1.0 on 2026-10-07T10:10:30+00:00: completeness 1.00; sense: Wikidata description; 2 live sources; review by codex: pass"},"score":1.0}}