NR1H4

Gene and receptor boundary

Require an explicit referent instead of inferring it from PHY.OBJ.

1. Does the originating registry record denote the NR1H4 gene, its protein product or a deliberately combined molecular concept? boundary
2. Which original identifier and organism support that interpretation? provenance

Alias disambiguation

Treat names such as FXR as lookup aids that require accession confirmation.

1. Which authoritative record connects the supplied NR1H4 or FXR label to the intended species and molecular form? provenance
2. What accession or sequence check excludes a similarly named receptor or an ortholog from another species? boundary

Reference sequence chain

Record versioned references and the evidence linking each transcript to its protein sequence.

1. Which versioned transcript and protein accessions describe the NR1H4 form being observed? definition
2. Does the experiment distinguish that isoform from other NR1H4 transcripts or proteins? measurement

Native versus engineered receptor

Document domain coverage, substitutions, deletions and tags before interpreting function.

1. Which DNA-binding and ligand-binding regions are present in the tested NR1H4 sequence? definition
2. Could a truncation, tag or substitution limit what this experiment establishes about endogenous NR1H4? boundary

Measured receptor abundance

Require tissue, cell identity, molecular analyte and normalization for abundance claims.

1. In which species, tissue and cell population was NR1H4 RNA or protein measured? measurement
2. Does the abundance reflect endogenous expression or an introduced construct, and how was it normalized? provenance

Receptor compartment evidence

Keep observed distribution separate from assumed activity.

1. What method establishes NR1H4 compartment distribution, and at what time after treatment? measurement
2. What additional evidence is required before interpreting the observed localization as transcriptional activation? boundary

Binding versus functional response

Preserve assay type, concentration and exposure when assessing ligand effects.

1. Was direct NR1H4 binding measured, or was engagement inferred from a functional response? measurement
2. Which ligand identity, concentration range, exposure duration and controls support the reported response? provenance

Target regulation evidence

Ask which target, regulatory sequence and molecular partners are supported in the tested context.

1. Which endogenous target or reporter was measured, and what evidence distinguishes direct regulation from an indirect response? measurement
2. Were RXR participation and relevant coregulators tested, inferred or left unresolved in this NR1H4 experiment? provenance

Alteration and functional consequence

Distinguish predicted effects from observed changes in abundance, binding or transcription.

1. Against which reference accession is the NR1H4 alteration described? definition
2. Which functional consequences were directly measured, and which remain predictions? measurement

Causal attribution controls

Use specificity and rescue evidence to bound claims about receptor-dependent phenotypes.

1. What independent perturbation, rescue or receptor-deficient control supports attribution of the observed effect to NR1H4? provenance
2. Which additional control is needed before using this result to choose a subsequent NR1H4 experiment? action

Check these first

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these check these first hold for the sense of NR1H4 this model covers, and on what evidence? provenance

Identifiers and schemes

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these identifiers and schemes hold for the sense of NR1H4 this model covers, and on what evidence? provenance

Real-world use

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these real-world use hold for the sense of NR1H4 this model covers, and on what evidence? provenance

Failure modes and hazards

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these failure modes and hazards hold for the sense of NR1H4 this model covers, and on what evidence? provenance

Neighbouring kinds and how to tell them apart

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these neighbouring kinds and how to tell them apart hold for the sense of NR1H4 this model covers, and on what evidence? provenance