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Research draft

ribosomal RNA

vr.tr.ribosomal-rna · PHY.MAT

Let an agent explain ribosomal RNA by type, structure, function, gene organisation and research uses such as microbial identification.

Thing Registry Physical world and living systems

Research draft, second pass

A second pass drafted this model: the structure a model of this thing needs, and what is known about it in the world. The line under this one says how the second half was obtained - researched against sources, or recalled without web access, in which case nothing here was read anywhere and every claim is a lead to verify. Unreviewed either way.

written by Claude from model knowledge without web access - no source was read, every claim is a lead to verify

Researched by: Claude

Purpose and description

Let an agent explain ribosomal RNA by type, structure, function, gene organisation and research uses such as microbial identification.

The RNA component of ribosomes, which forms their structural core and catalyses peptide bond formation; prokaryotes have 5S, 16S and 23S rRNAs and eukaryotes have 5S, 5.8S, 18S and 28S rRNAs, and 16S rRNA gene sequencing is widely used to identify bacteria.

What it is for: Protein synthesis, microbiology and evolutionary studies.

It can be explain rRNA types and ribosome structure; describe the catalytic role of rRNA; use 16S rRNA sequencing for identification; find rRNA sequence databases.

Distinguishing features

Structural and catalytic RNA

Most abundant RNA in cells

Highly conserved

Used as a phylogenetic marker

What it looks like

Not visible; shown in ribosome structures and sequence data.

How it is recognised

Sedimentation labels such as 16S and 23S

Folded RNA in ribosome structures

Messenger RNA carries coding information

Related models

is a kind of - category

non-coding RNA

is part of - complex

ribosome

catalyses - function

peptide bond formation

is used in - method

16S rRNA sequencing

In practice

Families and kinds

16S and 18S small-subunit rRNA

23S and 28S large-subunit rRNA

5S and 5.8S rRNA

mitochondrial and chloroplast rRNA

Standards and regulation

Laboratory biosafety rules for handling organisms

Failure modes and hazards

Overstating identification from partial sequences

Contamination in sequencing

Also called

self-splicing ribosomal RNA5S ribosomal RNA16S ribosomal RNA encoded by: VC_RS00270 VC_RS0027023S ribosomal RNA encoded by: VC_RS00280 VC_RS002805S ribosomal RNA encoded by: rrf VC_RS0028516S ribosomal RNA encoded by: VC_RS00735 VC_RS0073523S ribosomal RNA encoded by: VC_RS00745 VC_RS007455S ribosomal RNA encoded by: rrf VC_RS0075016S ribosomal RNA encoded by: VC_RS01505 VC_RS0150523S ribosomal RNA encoded by: VC_RS01520 VC_RS015205S ribosomal RNA encoded by: rrf VC_RS0152516S ribosomal RNA encoded by: VC_RS01940 VC_RS0194023S ribosomal RNA encoded by: VC_RS01955 VC_RS019555S ribosomal RNA encoded by: rrf VC_RS0196016S ribosomal RNA encoded by: VC_RS03580 VC_RS0358023S ribosomal RNA encoded by: VC_RS03590 VC_RS035905S ribosomal RNA encoded by: rrf VC_RS035955S ribosomal RNA encoded by: rrf VC_RS120155S ribosomal RNA encoded by: rrf VC_RS1202523S ribosomal RNA encoded by: VC_RS12030 VC_RS1203016S ribosomal RNA encoded by: VC_RS12045 VC_RS120455S ribosomal RNA encoded by: rrf VC_RS1326023S ribosomal RNA encoded by: VC_RS13265 VC_RS1326516S ribosomal RNA encoded by: VC_RS13290 VC_RS132905S ribosomal RNA encoded by: rrf VC_RS1329523S ribosomal RNA encoded by: VC_RS13300 VC_RS1330016S ribosomal RNA encoded by: VC_RS13320 VC_RS1332016S Ribosomal RNA encoded by: SAOUHSC_R0001 SAOUHSC_R000123S Ribosomal RNA encoded by: SAOUHSC_R0006 SAOUHSC_R00065S Ribosomal RNA encoded by: SAOUHSC_R00011 SAOUHSC_R000115S Ribosomal RNA encoded by: SAOUHSC_R00016 SAOUHSC_R0001616S Ribosomal RNA encoded by: SAOUHSC_R0002 SAOUHSC_R000223S Ribosomal RNA encoded by: SAOUHSC_R0007 SAOUHSC_R00075S Ribosomal RNA encoded by: SAOUHSC_R00012 SAOUHSC_R000125S Ribosomal RNA encoded by: SAOUHSC_R00013 SAOUHSC_R0001323S Ribosomal RNA encoded by: SAOUHSC_R0008 SAOUHSC_R000816S Ribosomal RNA encoded by: SAOUHSC_R0003 SAOUHSC_R00035S Ribosomal RNA encoded by: SAOUHSC_R00014 SAOUHSC_R0001423S Ribosomal RNA encoded by: SAOUHSC_R0009 SAOUHSC_R000916S Ribosomal RNA encoded by: SAOUHSC_R0004 SAOUHSC_R0004

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Where this came from

wikidata · CC0 1.0

Drafted structure

Bundle to layer to finding to question, as the second pass will find it: 4 bundles · 8 layers · 8 findings · 16 questions.

Types rRNA types.

Types differ by organism.

Which

Type.

Which

rRNA type.

  1. Which rRNA is meant, and is it prokaryotic, eukaryotic or organellar? definition
  2. What does the S value mean? definition

Genes

rRNA genes.

Genes

rRNA genes.

  1. How are rRNA genes organised and how many copies are there? provenance
  2. Which databases annotate them? provenance
Function Protein synthesis.

rRNA is catalytic.

Catalysis

Ribozyme.

Catalysis

Catalytic role.

  1. How does rRNA catalyse peptide bond formation? definition
  2. Which structural studies showed it? provenance

Antibiotics

Drug targets.

Antibiotics

Antibiotic targets.

  1. Which antibiotics act on bacterial ribosomes, in general terms? provenance
  2. Is the user asking about their own treatment? boundary
Methods Sequencing.

rRNA is a marker.

Sequencing

16S sequencing.

Sequencing

16S sequencing.

  1. How is 16S rRNA sequencing used to identify bacteria? definition
  2. What are its limits? boundary

Databases

Reference data.

Databases

Reference databases.

  1. Which reference databases hold rRNA sequences, such as SILVA? provenance
  2. How are they curated? provenance
Evolution Phylogeny.

rRNA shaped the tree of life.

Tree

Tree of life.

Tree

Tree of life.

  1. How did rRNA comparisons reveal the three domains of life? provenance
  2. Who proposed the domain system? provenance

Teaching

Explaining.

Teaching

Teaching.

  1. How can ribosome structure be explained with models? action
  2. Which structure viewers help? provenance

What the second pass must settle

  • Should each rRNA type be a separate entry?
  • How should sequence databases be linked?
  • How should gene copy variation be recorded?