kainate receptor

Resolved GluK components

Record current subunit names alongside accessions and historical labels. Human GluK1 corresponds to GRIK1. [IUPHAR/BPS GluK1](https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=450)

1. Which GluK subunits, species and accessions identify the receptor under consideration? definition
2. Which splice forms, editing states or engineered sequence changes were established in the preparation? provenance

Functional complex evidence

Record homomeric or heteromeric assignment, proposed stoichiometry and evidence that the components form the same functional complex.

1. What evidence distinguishes co-assembly from subunits merely being expressed in the same cell? provenance
2. Does the observation describe an isolated subunit, one receptor assembly or a mixture of receptor populations? boundary

Surface delivery evidence

Track total abundance and surface abundance separately; ligand-binding-site mutations have affected expression and surface delivery in recombinant kainate-receptor experiments. [Primary study](https://pubmed.ncbi.nlm.nih.gov/30302614/)

1. Which assay establishes plasma-membrane availability rather than total cellular subunit abundance? measurement
2. Could a reduced response reflect impaired surface delivery, and what measurement would distinguish it from altered gating? action

Compartment and auxiliary context

Record presynaptic, postsynaptic or other localization and whether NETO-family or other partners were tested, detected or experimentally supplied.

1. What evidence places this receptor population in the stated cell type and membrane compartment? provenance
2. Which auxiliary proteins are present, and is their association with this receptor demonstrated or assumed? provenance

Stimulus-linked state transitions

Associate kinetic measurements with ligand identity, concentration, application speed and pulse history instead of treating response amplitude as a complete functional description.

1. What ligand pulse and solution-exchange conditions produced the measured activation and desensitization? measurement
2. What paired-pulse or washout experiment distinguishes desensitization from persistent blockade or receptor loss? action

Variant-dependent permeation

Retain editing and assembly context when recording channel conductance; recombinant experiments demonstrate dependence on Q/R editing and subunit co-assembly. [Primary study](https://pubmed.ncbi.nlm.nih.gov/8730589/)

1. What conductance, reversal potential and permeability measurements were obtained, with which ionic solutions and voltages? measurement
2. Were relevant editing states determined directly, and how do they limit interpretation of calcium permeability or rectification? boundary

Assay-qualified pharmacology

Record ligand identity, concentration range, assay endpoint and receptor composition; keep binding affinity distinct from functional potency and efficacy.

1. Does the reported parameter measure binding, peak current, steady-state current or a downstream endpoint? definition
2. Which selectivity controls distinguish this ligand's kainate-receptor effect from activity at neighbouring glutamate receptors? action

Mechanism and transfer limits

Attach pharmacological, genetic and recording evidence to each functional claim, identifying whether it supports direct channel current, an indirect response or a proposed non-ionotropic mechanism.

1. Which independent controls establish that the observed effect depends on the identified kainate receptor population? provenance
2. What evidence separates ion flux through the receptor from downstream signalling or network-mediated effects? boundary
3. Which preparation differences must be resolved before transferring this finding to native tissue? action

Check these first

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these check these first hold for the sense of kainate receptor this model covers, and on what evidence? provenance

Kinds and varieties

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these kinds and varieties hold for the sense of kainate receptor this model covers, and on what evidence? provenance

Identifiers and schemes

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these identifiers and schemes hold for the sense of kainate receptor this model covers, and on what evidence? provenance

Real-world use

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these real-world use hold for the sense of kainate receptor this model covers, and on what evidence? provenance

Typical measurements

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these typical measurements hold for the sense of kainate receptor this model covers, and on what evidence? provenance

Failure modes and hazards

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these failure modes and hazards hold for the sense of kainate receptor this model covers, and on what evidence? provenance

Neighbouring kinds and how to tell them apart

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these neighbouring kinds and how to tell them apart hold for the sense of kainate receptor this model covers, and on what evidence? provenance