guide RNA

Guide role

Record the process in which the RNA provides recognition or editing information. RNA-editing guidance is an evidenced use of the term: [Structural basis of the RNA-editing cascade in trypanosome mitochondria](https://www.nature.com/articles/s41586-026-10831-x).

1. Which biological mechanism makes this RNA a guide, and what evidence establishes that role? definition
2. Does this record denote the RNA itself, an encoding construct, or a larger guide-containing complex? boundary

Sequence and variant record

Identify every chain, its annotated regions, terminal chemistry and modifications independently of target-based nicknames.

1. What complete sequences, chain boundaries, termini and chemical modifications define this guide variant? definition
2. Which accession, design version or supplier record establishes that identity, and was it independently verified? provenance

Target alignment

Preserve target identity, version, strand and guide-to-target pairing rather than recording only a gene name.

1. Which substrate sequence, reference version, coordinates and strand define the intended target? definition
2. How does the recognition region align to that substrate, including mismatches, gaps and relevant sample variants? measurement

Recognition eligibility

Treat adjacent motifs, substrate accessibility and pairing requirements as system-dependent conditions; do not assume a universal PAM requirement.

1. Which adjacent-sequence, pairing or substrate-state requirements apply to this exact guide-effector system? definition
2. What evidence supports target accessibility in the intended biological context? provenance

Chain and scaffold arrangement

Record single-chain or multipart architecture and scaffold identity. Cas9 guide format and folding distinctions are supported by [Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage](https://www.nature.com/articles/s41467-022-28137-7).

1. Which regions provide target recognition, partner binding and any additional guide function? definition
2. What evidence shows that the proposed chain arrangement and scaffold form a functional guide in this system? provenance

Assembly readiness

Distinguish predicted compatibility from demonstrated partner association and activity.

1. Which effector variant, companion RNA and processing steps are required for this guide to function? definition
2. Is the available RNA in the required mature and associated state, and what evidence supports proceeding with its intended use? action

Preparation quality

Record production route, lot identity, concentration basis and assays distinguishing intended guide from truncated RNA and other relevant impurities.

1. How was this preparation produced and purified, and which lot-specific records connect it to the intended molecular identity? provenance
2. What concentration, intact fraction and relevant impurity measurements are available, with methods and detection limits? measurement

Condition-dependent usability

Associate buffer, storage duration, temperature excursions and handling history with preparation-specific stability evidence.

1. What buffer, temperature, elapsed storage time and handling events characterize the material now? measurement
2. Which guide-specific acceptance criteria determine whether this preparation can be used, needs reassessment or should be rejected? action

Guide-dependent performance

Record measured responses with controls and conditions while keeping guide contribution distinct from effector, delivery and downstream biology.

1. Is the intended endpoint binding, cleavage, RNA editing, DNA editing, regulation or another specified response? definition
2. What controlled measurements support that endpoint, and in which effector, delivery and biological context were they obtained? measurement

Specificity evidence

Separate computational candidates, observed non-target responses and unexamined possibilities, including assay coverage and uncertainty.

1. Which unintended interactions or outcomes are predicted or observed, and what reference coverage and detection limits constrain those conclusions? measurement
2. Does the available evidence meet the intended application's activity and specificity criteria, or what additional evidence is required? action

Check these first

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these check these first hold for the sense of guide RNA this model covers, and on what evidence? provenance

Kinds and varieties

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these kinds and varieties hold for the sense of guide RNA this model covers, and on what evidence? provenance

Identifiers and schemes

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these identifiers and schemes hold for the sense of guide RNA this model covers, and on what evidence? provenance

Real-world use

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these real-world use hold for the sense of guide RNA this model covers, and on what evidence? provenance

Typical measurements

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these typical measurements hold for the sense of guide RNA this model covers, and on what evidence? provenance

Failure modes and hazards

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these failure modes and hazards hold for the sense of guide RNA this model covers, and on what evidence? provenance

Neighbouring kinds and how to tell them apart

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these neighbouring kinds and how to tell them apart hold for the sense of guide RNA this model covers, and on what evidence? provenance