{ "model": { "rank": 6002, "code": "thing-q484940", "model_id": "vr.tr.l-isoleucine", "name": "L-isoleucine", "purpose": "Enable an AI agent to recognise L-isoleucine, assess the identity and condition of material containing it, and decide whether that material is suitable for a specified use.", "family": "Thing Registry", "category": "Physical world and living systems", "status": "research-draft", "kind": "thing", "plane": "PHY", "domain": "PHY.MAT", "industry": "", "version": "", "url": "/models/thing/q484940/", "tier": 2, "score": 63, "payload": { "layer": "wikidata", "aliases": [], "aliasCount": 0, "merged": 0, "knownIn": 63, "facets": null, "markers": [], "lexicalClass": "", "senseRank": null, "alsoRegisteredAs": null, "source": { "dataset": "wikidata", "item": "Q484940", "url": "https://www.wikidata.org/wiki/Q484940", "license": "CC0 1.0" } }, "research": { "vercy": "1.0-draft", "publication": { "status": "research-draft", "adjudicationStatus": "unreviewed", "publishableCanonical": false, "generatedAt": "2026-09-07T10:42:06Z", "providers": [ "Codex", "Grok" ], "missingProviders": [], "pass": 2, "cost": { "grok": { "usd": 0.00807942, "turns": 1, "seconds": 152.8, "error": null }, "codex": { "seconds": 228.6, "error": "Reading additional input from stdin...\nOpenAI Codex v0.153.3\n--------\nworkdir: R:\\02_PROJECTS\\02_Meta_Models_Platforms\\Ver.cy\\current\\thing-registry-backlog\nmodel: gpt-6-astra\nprovider: openai\napproval: never\nsandbox: read-only\nreasoning effort: none\nreasoning summaries: none\nsession id: 01a07b72-882d-78b0-b115-83139e496f13\n--------\nuser\nYou are drafting a Vercy meta-model for one registered thing. Answer as JSON only, no prose around it.\n\nThing: L-isoleucine\nRegistry id: vr.tr.l-isoleucine\nPlane / domain: PHY / PHY.MAT\nRegistry definition: (none recorded)\nNames folded into this entry: (none)\n" } } }, "metaModel": { "id": "THING-Q484940", "registryId": "vr.tr.l-isoleucine", "name": "L-isoleucine", "version": "0.1.0-research.1", "entryKind": "thing", "family": "Thing Registry", "domain": [ "PHY.MAT" ], "status": "research-draft" }, "canonicalUrl": "https://ver.cy/models/thing/q484940/", "model": { "registry_id": "vr.tr.l-isoleucine", "name": "L-isoleucine", "purpose": "Enable an AI agent to recognise L-isoleucine, assess the identity and condition of material containing it, and decide whether that material is suitable for a specified use.", "definition": "L-Isoleucine is the (2S,3S)-2-amino-3-methylpentanoic acid enantiomer of the branched-chain amino acid isoleucine, an essential proteinogenic residue encoded by AUU, AUC and AUA, distinguished from its allo and D stereoisomers by the (S) configuration at both C2 and C3.", "scope_statement": "This model owns the chemical identity of vr.tr.l-isoleucine and the evidence needed to assess its stereochemistry, material form, quantity, purity, handling condition and suitability for use; mixtures, organisms and processes are linked contexts.", "in_scope": [ "L-isoleucine molecular connectivity and stereochemical identity", "Free amino acid and its condition-dependent protonation states", "Identification and quantification in isolated material or a specified matrix", "Chemical purity, stereoisomer composition and relevant contamination", "Material condition, preparation history and suitability for a declared application" ], "out_of_scope": [ "Leucine, valine and other amino acids as independently modelled substances", "D-isoleucine and alloisoleucine as independently modelled stereoisomers", "Peptides, proteins and covalently modified isoleucine derivatives", "Complete formulations, foods and culture media containing L-isoleucine", "Patient diagnosis, dietary requirements and treatment decisions", "Manufacturing equipment and complete fermentation or synthesis processes" ], "distinguishing_features": [ "Require connectivity matching 2-amino-3-methylpentanoic acid and configuration (2S,3S), rather than accepting an unspecified isoleucine label. [ChEBI](https://www.ebi.ac.uk/chebi/CHEBI%3A17191)", "Use structural or validated separation evidence to distinguish leucine and other constitutional isomers; molecular formula or mass alone does not establish L-isoleucine identity.", "Require a method capable of resolving relevant stereoisomers before distinguishing L-isoleucine from D-isoleucine or alloisoleucine; an achiral identity assay alone is insufficient.", "Determine whether the measurement represents free L-isoleucine or material released from peptides and proteins during sample preparation.", "Distinguish a change in protonation from a change in covalent identity: the zwitterion retains L-isoleucine identity while expressing charged amino and carboxyl groups. [ChEBI zwitterion record](https://www.ebi.ac.uk/chebi/searchId.do?chebiId=58045)" ], "characteristics": [ { "name": "Identity evidence", "kind": "relation", "unit_or_values": "Assigned structure linked to reference material, analytical result and method", "why_it_matters": "A supplier name or shared molecular formula cannot resolve structural and stereochemical alternatives." }, { "name": "Stereochemical composition", "kind": "measurement", "unit_or_values": "Mole fraction or percentage of individually resolved isoleucine stereoisomers; unresolved fraction stated", "why_it_matters": "Chemical assay and stereochemical purity answer different questions about the material." }, { "name": "Chemical presentation", "kind": "category", "unit_or_values": "Isolated free amino acid, solution, mixture component or separately identified salt presentation", "why_it_matters": "Presentation determines which mass basis, counterions and preparation conditions must accompany an assessment." }, { "name": "Solution conditions", "kind": "measurement", "unit_or_values": "pH, temperature in °C, solvent composition and ionic strength where relevant", "why_it_matters": "Protonation, dissolution and analytical behaviour must be interpreted under recorded conditions." }, { "name": "L-isoleucine content", "kind": "measurement", "unit_or_values": "mg, mmol, mg/mL, mmol/L or mass percentage, with explicit sample and moisture basis", "why_it_matters": "Supports preparation and comparison without confusing material mass with actual L-isoleucine amount." }, { "name": "Water and volatile content", "kind": "measurement", "unit_or_values": "Mass percentage with method; water distinguished from total loss on drying", "why_it_matters": "Moisture and residual volatiles can alter the relationship between weighed mass and amino acid content." }, { "name": "Application-relevant impurities", "kind": "measurement", "unit_or_values": "Identity and concentration of specified impurities, with detection and quantification limits", "why_it_matters": "Other amino acids, process residues and contamination can invalidate a use even when identity is confirmed." }, { "name": "Material condition", "kind": "state", "unit_or_values": "Documented appearance, dissolution state, exposure history and retest status", "why_it_matters": "Condition changes determine whether earlier analytical evidence still supports use." }, { "name": "Intended-use qualification", "kind": "relation", "unit_or_values": "Material linked to application, specification version, supporting evidence and disposition", "why_it_matters": "Suitability as a reference standard, culture ingredient or formulation input cannot be inferred from chemical identity alone." } ], "affordances": [ "Identify material as L-isoleucine or flag unresolved structural and stereochemical alternatives.", "Calculate a required material quantity from measured L-isoleucine content and its stated basis.", "Prepare a solution under recorded conditions and verify its concentration and dissolution state.", "Select complementary identity, stereoisomer and impurity measurements for a proposed use.", "Accept, quarantine or request retesting of a lot against an applicable specification.", "Link L-isoleucine consumption or incorporation to a reaction, culture or formulation record." ] }, "sources": [ { "id": "pubchem-ile", "title": "Isoleucine, Compound Summary CID 6306", "url": "https://pubchem.ncbi.nlm.nih.gov/compound/Isoleucine", "what_it_supports": "Canonical identity, formula, stereochemistry, CAS, InChI, melting point, solubility, and encoded-codon facts.", "url_status": "live" }, { "id": "who-trs-970-a3", "title": "WHO Technical Report Series 970, Annex 3: Specifications for isoleucine", "url": "", "what_it_supports": "Pharmacopoeial identity tests, assay, specific rotation, and impurity limits used as a pharmaceutical substance.", "url_status": "dead" }, { "id": "efsa-2015-ile", "title": "Scientific Opinion on the safety and efficacy of L-isoleucine produced by Escherichia coli FERM ABP-10641 for all animal species", "url": "https://efsa.onlinelibrary.wiley.com/doi/10.2903/j.efsa.2015.4239", "what_it_supports": "Feed-additive use, typical dietary inclusion, manufacturing organism, and EU regulatory framing.", "url_status": "blocked" }, { "id": "iupac-goldbook-ile", "title": "Isoleucine (Ile, I)", "url": "https://goldbook.iupac.org/terms/view/I03275", "what_it_supports": "IUPAC recommended name and proteinogenic-amino-acid status.", "url_status": "blocked" } ], "structure": { "bundles": [ { "id": "molecular-and-stereochemical-identity", "name": "Molecular and stereochemical identity", "description": "Evidence that the recorded substance has L-isoleucine connectivity and stereochemistry.", "rationale": "Closely related amino acids and isoleucine stereoisomers can satisfy incomplete identity checks.", "layers": [ { "id": "connectivity-recognition", "name": "Connectivity recognition", "description": "Establish the amino acid skeleton independently of naming conventions.", "findings": [ { "id": "isoleucine-connectivity", "name": "Isoleucine connectivity", "description": "Record evidence supporting the assigned connectivity and excluding relevant constitutional isomers.", "questions": [ { "text": "What structural evidence establishes 2-amino-3-methylpentanoic acid rather than leucine or another isomer?", "kind": "definition", "id": "isoleucine-connectivity-q01" }, { "text": "Which reference material and analytical method support the identity assignment?", "kind": "provenance", "id": "isoleucine-connectivity-q02" } ] } ] }, { "id": "stereochemical-recognition", "name": "Stereochemical recognition", "description": "Resolve both stereocentres and identify limits of the available evidence.", "findings": [ { "id": "two-centre-configuration", "name": "Two-centre configuration", "description": "Require explicit support for the L-isoleucine configuration and separately account for unresolved stereoisomers.", "questions": [ { "text": "What evidence establishes the (2S,3S) assignment at both stereocentres?", "kind": "measurement", "id": "two-centre-configuration-q01" }, { "text": "Does the method distinguish D-isoleucine and the relevant alloisoleucine stereoisomers at the required limits?", "kind": "boundary", "id": "two-centre-configuration-q02" } ] } ] } ] }, { "id": "chemical-form-and-dissolution", "name": "Chemical form and dissolution", "description": "The form in which L-isoleucine is present and the conditions governing its preparation.", "rationale": "A free amino acid, its solution species and a salt presentation require different accounting and preparation evidence.", "layers": [ { "id": "protonation-and-presentation", "name": "Protonation and presentation", "description": "Distinguish reversible solution speciation from separately constituted material.", "findings": [ { "id": "form-assignment", "name": "Form assignment", "description": "Record the material presentation, counterions where present and conditions supporting any protonation assignment.", "questions": [ { "text": "Is the material an isolated free amino acid, a solution species or a salt with an identified counterion?", "kind": "definition", "id": "form-assignment-q01" }, { "text": "What measured pH and solvent conditions support the assigned protonation state?", "kind": "measurement", "id": "form-assignment-q02" }, { "text": "Does the presentation require a linked salt or mixture model while retaining L-isoleucine as the component identity?", "kind": "boundary", "id": "form-assignment-q03" } ] } ] }, { "id": "dissolution-and-preparation", "name": "Dissolution and preparation", "description": "Establish whether the required amount is dissolved under the actual preparation conditions.", "findings": [ { "id": "verified-solution-state", "name": "Verified solution state", "description": "Record solvent, temperature, pH adjustment and evidence of complete dissolution or precipitation.", "questions": [ { "text": "At the intended concentration, solvent composition, pH and temperature, is the L-isoleucine fully dissolved?", "kind": "measurement", "id": "verified-solution-state-q01" }, { "text": "What preparation or concentration adjustment is supported if undissolved material or later precipitation is observed?", "kind": "action", "id": "verified-solution-state-q02" } ] } ] } ] }, { "id": "content-and-analytical-selectivity", "name": "Content and analytical selectivity", "description": "Quantification of L-isoleucine with explicit analyte boundaries, sample basis and method limitations.", "rationale": "A reported amino acid concentration may combine isomers, include hydrolysis products or use an incompatible mass basis.", "layers": [ { "id": "analyte-boundary", "name": "Analyte boundary", "description": "Define what the measurement actually counts as L-isoleucine.", "findings": [ { "id": "free-versus-released-content", "name": "Free versus released content", "description": "Separate free L-isoleucine measurements from results obtained after cleavage of peptide-bound material.", "questions": [ { "text": "Does the result quantify free L-isoleucine, hydrolytically released isoleucine or an unresolved combined signal?", "kind": "definition", "id": "free-versus-released-content-q01" }, { "text": "What extraction, hydrolysis or derivatisation steps could change recovery or stereochemical interpretation?", "kind": "provenance", "id": "free-versus-released-content-q02" } ] } ] }, { "id": "quantitative-confidence", "name": "Quantitative confidence", "description": "Relate the measured amount to selective detection and a defensible calculation basis.", "findings": [ { "id": "selective-content-result", "name": "Selective content result", "description": "Record calibration, interference checks, uncertainty and the mass or volume basis of the result.", "questions": [ { "text": "How does the method exclude contributions from leucine, other stereoisomers and matrix interference?", "kind": "measurement", "id": "selective-content-result-q01" }, { "text": "Is content reported as received, on a dry basis or as free-amino-acid equivalent, and what corrections were applied?", "kind": "measurement", "id": "selective-content-result-q02" }, { "text": "What amount of this material supplies the requested L-isoleucine quantity within the required uncertainty?", "kind": "action", "id": "selective-content-result-q03" } ] } ] } ] }, { "id": "lot-purity-and-condition", "name": "Lot purity and condition", "description": "Evidence that a particular L-isoleucine lot remains sufficiently pure and fit for evaluation.", "rationale": "Confirmed molecular identity does not establish lot quality or the continuing validity of a certificate.", "layers": [ { "id": "impurity-and-origin-evidence", "name": "Impurity and origin evidence", "description": "Connect lot origin to relevant impurity measurements.", "findings": [ { "id": "route-relevant-purity", "name": "Route-relevant purity", "description": "Record origin and purification evidence to justify which related amino acids, residues and contaminants require assessment.", "questions": [ { "text": "What production and purification history is documented for this L-isoleucine lot?", "kind": "provenance", "id": "route-relevant-purity-q01" }, { "text": "Which related amino acids, stereoisomers, process residues or contaminants were measured, and with what limits?", "kind": "measurement", "id": "route-relevant-purity-q02" } ] } ] }, { "id": "condition-and-retest", "name": "Condition and retest", "description": "Assess whether storage and preparation history leave the analytical qualification applicable.", "findings": [ { "id": "continued-lot-validity", "name": "Continued lot validity", "description": "Connect moisture, appearance, exposure and solution history to evidence-based retest decisions.", "questions": [ { "text": "Have water content, appearance, dissolution behaviour or assay changed relative to the qualified lot?", "kind": "measurement", "id": "continued-lot-validity-q01" }, { "text": "Which documented storage or preparation limits were exceeded, and what evidence supports retesting or continued use?", "kind": "action", "id": "continued-lot-validity-q02" } ] } ] } ] }, { "id": "application-fit-and-disposition", "name": "Application fit and disposition", "description": "Translate L-isoleucine identity and material evidence into a decision for a declared application.", "rationale": "Requirements for an analytical reference, culture nutrient or formulation ingredient differ even for the same chemical identity.", "layers": [ { "id": "role-specific-qualification", "name": "Role-specific qualification", "description": "Connect the intended role to the necessary analytical and quality evidence.", "findings": [ { "id": "declared-use-requirements", "name": "Declared use requirements", "description": "Record the proposed role and its acceptance requirements without inferring suitability from a grade label.", "questions": [ { "text": "Will the L-isoleucine serve as an analytical reference, reaction substrate, culture ingredient or formulation input?", "kind": "definition", "id": "declared-use-requirements-q01" }, { "text": "Which application-specific requirements for assay, stereoisomers, impurities and contamination apply?", "kind": "boundary", "id": "declared-use-requirements-q02" }, { "text": "Which specification and lot-specific evidence substantiate the claimed grade?", "kind": "provenance", "id": "declared-use-requirements-q03" } ] } ] }, { "id": "use-decision", "name": "Use decision", "description": "Determine the supported material action and its boundary with the receiving process or product.", "findings": [ { "id": "supported-disposition", "name": "Supported disposition", "description": "Record whether available evidence supports use, restricted use, quarantine or further testing.", "questions": [ { "text": "Do the measured identity, content, impurity profile and condition support the intended use, or is further testing required?", "kind": "action", "id": "supported-disposition-q01" }, { "text": "Which handling and preparation instructions are supported by the lot documentation and receiving process?", "kind": "action", "id": "supported-disposition-q02" }, { "text": "At what point must a decision be transferred to a formulation, biological system or clinical model?", "kind": "boundary", "id": "supported-disposition-q03" } ] } ] } ] }, { "id": "evidence-and-external-alignment", "name": "Evidence and external alignment", "description": "What the world already says about this thing, gathered so the model can be checked against it.", "rationale": "A model that cannot be lined up against existing standards, identifiers and practice cannot be adopted by anyone who already uses them.", "layers": [ { "id": "reported-evidence", "name": "Reported evidence", "description": "Findings from the breadth pass, kept separate from the structural claims.", "findings": [ { "id": "evidence-kinds", "name": "Kinds and varieties", "description": "Reported by the breadth pass; each item needs checking against its source before it becomes normative.", "evidence": [ "Free L-isoleucine (crystalline amino-acid ingredient)", "Protein-bound L-isoleucine residues in peptides and proteins", "Pharmaceutical-grade L-isoleucine (USP/Ph. Eur. monograph substance)", "Feed-grade L-isoleucine (amino-acid supplement for livestock diets)", "Food-grade L-isoleucine (flavour enhancer / dietary-supplement ingredient)", "Isotopically labelled L-isoleucine (13C, 15N, or deuterated research reagents)", "L-isoleucine salts and esters (e.g. hydrochloride, ethyl ester)", "Cell-culture media L-isoleucine (sterile, endotoxin-controlled)" ], "questions": [ { "id": "evidence-kinds-q01", "text": "Which of these kinds and varieties hold for the sense of L-isoleucine this model covers, and on what evidence?", "kind": "provenance" } ] }, { "id": "evidence-identifiers", "name": "Identifiers and schemes", "description": "Reported by the breadth pass; each item needs checking against its source before it becomes normative.", "evidence": [ { "scheme": "CAS Registry Number", "value_or_pattern": "73-32-5", "note": "L-isoleucine specifically; racemic isoleucine is 443-79-8; D-isoleucine is 319-78-8." }, { "scheme": "PubChem CID", "value_or_pattern": "6306", "note": "L-isoleucine; allo-L-isoleucine is CID 99288." }, { "scheme": "ChEBI", "value_or_pattern": "CHEBI:17191", "note": "L-isoleucine." }, { "scheme": "IUPAC InChIKey", "value_or_pattern": "AGPKZVBTJJNPAG-WHFBIAKZSA-N", "note": "Stereospecific; the InChI encodes (2S,3S)." }, { "scheme": "Wikidata", "value_or_pattern": "Q484940", "note": "item L-isoleucine." }, { "scheme": "EC / EINECS", "value_or_pattern": "200-798-2", "note": "European inventory number for L-isoleucine." }, { "scheme": "UNII", "value_or_pattern": "04S9037O6P", "note": "FDA Unique Ingredient Identifier." }, { "scheme": "IUPAC / three-letter / one-letter codes", "value_or_pattern": "L-isoleucine / Ile / I", "note": "Protein sequence notation; codon set AUU, AUC, AUA." } ], "questions": [ { "id": "evidence-identifiers-q01", "text": "Which of these identifiers and schemes hold for the sense of L-isoleucine this model covers, and on what evidence?", "kind": "provenance" } ] }, { "id": "evidence-standards-and-regulation", "name": "Standards and regulation", "description": "Reported by the breadth pass; each item needs checking against its source before it becomes normative.", "evidence": [ "United States Pharmacopeia (USP) monograph Isoleucine - identity, assay, specific rotation, related substances.", "European Pharmacopoeia (Ph. Eur.) monograph Isoleucine (01/2008:0770) - same class of tests as USP.", "Japanese Pharmacopoeia (JP) Isoleucine - JP-grade pharmaceutical substance.", "WHO Technical Report Series 970 Annex 3 specifications for isoleucine (pharmaceutical quality).", "Commission Regulation (EU) No 1831/2003 and related EFSA opinions - L-isoleucine as a feed additive (functional group: amino acids, their salts and analogues).", "FDA 21 CFR (food additive / GRAS pathway for amino acids used in food and dietary supplements; isoleucine listed among amino acids used in food).", "Codex Alimentarius / FAO-WHO amino-acid scoring (IAAO / DIAAS frameworks treat isoleucine as an indispensable amino acid with a scoring pattern).", "FCC (Food Chemicals Codex) specification for L-isoleucine as a food ingredient." ], "questions": [ { "id": "evidence-standards-and-regulation-q01", "text": "Which of these standards and regulation hold for the sense of L-isoleucine this model covers, and on what evidence?", "kind": "provenance" } ] }, { "id": "evidence-real-world-use", "name": "Real-world use", "description": "Reported by the breadth pass; each item needs checking against its source before it becomes normative.", "evidence": [ "Essential amino-acid residue in dietary protein; humans cannot synthesise it and must obtain it from food or supplements.", "Crystalline feed additive, typically produced by fermentation (E. coli or Corynebacterium), blended into swine, poultry and aquaculture diets to meet ileal digestible isoleucine requirements without overfeeding intact protein.", "Component of parenteral nutrition amino-acid solutions and of medical foods for maple-syrup-urine-disease (MSUD) management, where isoleucine intake is tightly titrated.", "Ingredient in sports-nutrition BCAA powders (leucine : isoleucine : valine blends, commonly 2:1:1).", "Cell-culture media component; mammalian cells require exogenous L-isoleucine.", "Starting material or chiral building block in peptide synthesis and some small-molecule APIs.", "Flavour / savoury note in some processed foods (bitter-sweet branched-chain amino acid taste)." ], "questions": [ { "id": "evidence-real-world-use-q01", "text": "Which of these real-world use hold for the sense of L-isoleucine this model covers, and on what evidence?", "kind": "provenance" } ] }, { "id": "evidence-measurements", "name": "Typical measurements", "description": "Reported by the breadth pass; each item needs checking against its source before it becomes normative.", "evidence": [ { "quantity": "Molecular mass", "typical_range": "131.17 (monoisotopic 131.0946)", "unit": "g mol−1" }, { "quantity": "Specific optical rotation [α]D20 (c = 4 in 6 M HCl, Ph. Eur./WHO)", "typical_range": "+38.0 to +42.0 (often about +40)", "unit": "° (degrees)" }, { "quantity": "Aqueous solubility (25 °C)", "typical_range": "about 30-40 (sparingly to freely soluble depending on pH)", "unit": "g L−1" }, { "quantity": "Isoelectric point (pI)", "typical_range": "about 6.0", "unit": "pH units" }, { "quantity": "Melting / decomposition point", "typical_range": "about 284-288 (decomposes)", "unit": "°C" }, { "quantity": "Assay (dried basis, pharmacopoeial)", "typical_range": "98.5-101.5", "unit": "%" }, { "quantity": "Human adult estimated average requirement (WHO/FAO/UNU 2007 scoring pattern, isoleucine)", "typical_range": "20 (mg per g protein) or about 20 mg kg−1 d−1 depending on the reference used", "unit": "mg g−1 protein or mg kg−1 d−1" }, { "quantity": "Plasma concentration (healthy fasting adult)", "typical_range": "about 40-100", "unit": "µmol L−1" } ], "questions": [ { "id": "evidence-measurements-q01", "text": "Which of these typical measurements hold for the sense of L-isoleucine this model covers, and on what evidence?", "kind": "provenance" } ] }, { "id": "evidence-failure-modes-and-hazards", "name": "Failure modes and hazards", "description": "Reported by the breadth pass; each item needs checking against its source before it becomes normative.", "evidence": [ "Maple syrup urine disease (branched-chain α-ketoacid dehydrogenase deficiency): accumulation of isoleucine, leucine and valine and their keto-acids; isoleucine excess contributes to metabolic decompensation.", "Dietary imbalance of branched-chain amino acids: excess leucine can antagonise isoleucine (and valine) utilisation; low dietary isoleucine relative to leucine causes reduced feed intake in pigs and poultry.", "MSUD medical-food error: too little isoleucine in a restricted diet produces an isoleucine-deficiency rash (acrodermatitis-like eruption) and growth failure; too much precipitates ketoacidosis.", "Stereochemical contamination: allo-isoleucine and D-isoleucine fail pharmacopoeial specific-rotation and related-substance tests and are metabolically non-equivalent.", "Fermentation-process impurities (endotoxin, residual host DNA/protein, other amino acids) if the feed- or pharma-grade purification is incomplete.", "Hygroscopicity and Maillard browning when stored with reducing sugars at elevated temperature/humidity, degrading assay and generating off-colours.", "Not an acute toxicant at food-use levels; very high supplemental BCAA intakes can raise plasma ammonia and compete with other large-neutral amino acids at the blood-brain barrier." ], "questions": [ { "id": "evidence-failure-modes-and-hazards-q01", "text": "Which of these failure modes and hazards hold for the sense of L-isoleucine this model covers, and on what evidence?", "kind": "provenance" } ] }, { "id": "evidence-regional-variation", "name": "Regional variation", "description": "Reported by the breadth pass; each item needs checking against its source before it becomes normative.", "evidence": [ "English and most pharmacopoeias use \"isoleucine\" / \"L-isoleucine\"; Japanese JP uses イソロイシン (isoroi shin).", "Feed-grade crystalline L-isoleucine is widely used in East-Asian and European pig and poultry formulation; uptake in North-American commercial diets is later and more leucine-driven.", "MSUD newborn-screening cut-offs and whether allo-isoleucine is the primary marker vary by programme (US state labs vs many EU programmes).", "Food-additive listing: EU treats crystalline L-isoleucine primarily as a feed additive and as a food-supplement amino acid; US lists amino acids including isoleucine under food-additive / GRAS and USP dietary-ingredient frameworks rather than an E-number." ], "questions": [ { "id": "evidence-regional-variation-q01", "text": "Which of these regional variation hold for the sense of L-isoleucine this model covers, and on what evidence?", "kind": "provenance" } ] }, { "id": "evidence-neighbours", "name": "Neighbouring kinds and how to tell them apart", "description": "Reported by the breadth pass; each item needs checking against its source before it becomes normative.", "evidence": [ { "name": "D-isoleucine", "difference": "Opposite configuration at both stereocentres (2R,3R). Separated by specific optical rotation (L is strongly dextrorotatory in HCl; D is levorotatory) and by chiral HPLC or GC of derivatives." }, { "name": "L-allo-isoleucine (2S,3R)", "difference": "Epimer at C3; diagnostic plasma marker of MSUD. Separated from L-isoleucine by ion-exchange or reversed-phase HPLC of amino-acid derivatives, and by 13C NMR / optical rotation." }, { "name": "D-allo-isoleucine (2R,3S)", "difference": "The remaining diastereomer of 2-amino-3-methylpentanoic acid. Same chromatographic and polarimetric tests as for the other three stereoisomers." }, { "name": "L-leucine", "difference": "Isomeric branched-chain amino acid (2-amino-4-methylpentanoic acid, one stereocentre). Distinguished by ninhydrin/ion-exchange elution order, mass spectrometry of isobaric fragments after derivatisation, and by side-chain NMR (isoleucine has a sec-butyl chain with a chiral β-carbon)." }, { "name": "L-valine", "difference": "Smaller branched-chain amino acid (2-amino-3-methylbutanoic acid). Separated by amino-acid analysis (shorter retention than Ile) and by molecular mass (117 vs 131)." }, { "name": "Norleucine (2-aminohexanoic acid)", "difference": "Straight-chain isomer sometimes used as an internal standard; co-elutes with isoleucine in some ninhydrin systems. Distinguised by HPLC method selection or by MS (same C6H13NO2 formula - needs chromatography, not mass alone)." } ], "questions": [ { "id": "evidence-neighbours-q01", "text": "Which of these neighbouring kinds and how to tell them apart hold for the sense of L-isoleucine this model covers, and on what evidence?", "kind": "provenance" } ] } ] } ] } ] }, "openQuestions": [ "Does an existing Vercy world model already own L-isoleucine, and what registry conventions govern links to its salts, protonation states and stereoisomers?", "Which reference methods and materials adequately resolve L-isoleucine from leucine and all relevant isoleucine stereoisomers in the intended matrices?", "Which authoritative specifications and impurity limits apply to each intended grade, application and jurisdiction?", "What measured solubility and stability evidence supports the intended solvent, pH, temperature, concentration and storage conditions?", "How should results after hydrolysis or derivatisation be qualified when recovery and stereochemical preservation have not been established?" ], "statistics": { "bundles": 6, "layers": 11, "findings": 18, "questions": 32 } }, "draft": { "generator": "vr.draft.v3", "status": "draft-generated", "researched": false, "archetype": "chemical substance or material", "method": "Written from the archetype playbook - what this kind of thing needs beyond identity and provenance - and from the structure that recurred across 6,333 models already researched by two engines. Applied to this entry by rule. No source was read for this thing and no claim here is researched. This entry carries no facets of its own, so they were inferred from its domain - a guess about a whole domain applied to one thing.", "facetsInferred": true, "nextPass": "A researcher replaces this draft with a sourced specification. Treat every sentence below as a proposal to argue with.", "purpose": "Give an agent a durable, checkable way to recognise a L-isoleucine, record what state it is in, and decide what may be done with it.", "whatItIs": "Enable an AI agent to recognise L-isoleucine, assess the identity and condition of material containing it, and decide whether that material is suitable for a specified use.", "characteristics": { "substance": "material", "origin": "natural", "agency": "inert", "mobility": "varies" }, "whatYouCanDoWithIt": [ "observed and measured", "consumed or used up" ], "distinguishingFeatures": [ "Described in 63 Wikipedia languages, which is a measure of how widely the thing is known, not of how important it is." ], "openQuestionsForResearch": [ "Which of the bundles below does a real task actually need, and which are ceremony?", "What does this thing have that the facets do not capture at all?", "Which neighbouring kind is most often confused with a L-isoleucine, and on what evidence are they told apart?" ], "whatItIsMadeOf": "matter you can touch", "physicalCharacter": [ "Mobility varies between examples.", "Does nothing on its own; everything it does, something else did to it.", "These come from the domain this entry sits in rather than from the entry itself, so treat them as a first guess about the whole domain applied to one thing." ], "whatCanBeDoneWithIt": [ "observe it, measure it, record its state", "use it up, and then need more of it" ], "howItIsRecognised": [ "Not recognisable by sight in any reliable way. Appearance varies with grade, form and additives; identification is by label, by documentation, or by assay." ], "relatedModels": [], "standing": "Described in 63 Wikipedia languages, which measures how widely it is written about rather than how important or how common it is.", "structure": { "bundles": [ { "id": "identity-and-classification", "name": "Identity, naming and classification", "description": "How an agent tells one L-isoleucine from another, and a L-isoleucine from things that resemble it.", "rationale": "Recognition comes before every other claim. Without stable identity nothing else in the model can be trusted to be about the same thing twice.", "layers": [ { "id": "naming-and-identifiers", "name": "Names and identifiers", "description": "The names this thing goes by and the identifiers that survive translation and time.", "findings": [ { "id": "preferred-name-and-aliases", "name": "Preferred name, aliases and local names", "description": "Which name to use, which names mean the same thing, and which merely sound similar.", "questions": [ { "id": "preferred-name-and-aliases-q01", "text": "What identifies and describes the name of a L-isoleucine, and in what units or vocabulary?", "kind": "definition", "answer_data": [ "identifiers", "types and classes", "values with units", "explicit unknowns" ] }, { "id": "preferred-name-and-aliases-q02", "text": "Who or what asserted this about the name of a L-isoleucine, by which method, and when was it true?", "kind": "provenance", "answer_data": [ "authority", "method", "evidence", "event time", "knowledge time" ] }, { "id": "preferred-name-and-aliases-q03", "text": "What may an agent decide or do once the name of a L-isoleucine is known, and what must it refuse?", "kind": "action", "answer_data": [ "permitted actions", "preconditions", "refusals", "escalation" ] } ] }, { "id": "stable-identifiers", "name": "Stable identifiers and external keys", "description": "Identifiers that keep pointing at this kind of thing across systems and languages.", "questions": [ { "id": "stable-identifiers-q01", "text": "What identifies and describes an identifier for a L-isoleucine, and in what units or vocabulary?", "kind": "definition", "answer_data": [ "identifiers", "types and classes", "values with units", "explicit unknowns" ] }, { "id": "stable-identifiers-q02", "text": "Who or what asserted this about an identifier for a L-isoleucine, by which method, and when was it true?", "kind": "provenance", "answer_data": [ "authority", "method", "evidence", "event time", "knowledge time" ] }, { "id": "stable-identifiers-q03", "text": "What may an agent decide or do once an identifier for a L-isoleucine is known, and what must it refuse?", "kind": "action", "answer_data": [ "permitted actions", "preconditions", "refusals", "escalation" ] } ] } ] }, { "id": "classification-and-granularity", "name": "Classification and granularity", "description": "Where a L-isoleucine sits among kinds, and how finely a task needs to cut it.", "findings": [ { "id": "kind-and-parents", "name": "Kind, parents and neighbouring kinds", "description": "The classes this thing belongs to and the ones it is next to.", "questions": [ { "id": "kind-and-parents-q01", "text": "What identifies and describes the kind of a L-isoleucine, and in what units or vocabulary?", "kind": "definition", "answer_data": [ "identifiers", "types and classes", "values with units", "explicit unknowns" ] }, { "id": "kind-and-parents-q02", "text": "Who or what asserted this about the kind of a L-isoleucine, by which method, and when was it true?", "kind": "provenance", "answer_data": [ "authority", "method", "evidence", "event time", "knowledge time" ] }, { "id": "kind-and-parents-q03", "text": "What may an agent decide or do once the kind of a L-isoleucine is known, and what must it refuse?", "kind": "action", "answer_data": [ "permitted actions", "preconditions", "refusals", "escalation" ] } ] }, { "id": "distinguishing-features", "name": "Distinguishing features", "description": "What separates a L-isoleucine from the things most often confused with it.", "questions": [ { "id": "distinguishing-features-q01", "text": "What identifies and describes what distinguishes a L-isoleucine, and in what units or vocabulary?", "kind": "definition", "answer_data": [ "identifiers", "types and classes", "values with units", "explicit unknowns" ] }, { "id": "distinguishing-features-q02", "text": "Who or what asserted this about what distinguishes a L-isoleucine, by which method, and when was it true?", "kind": "provenance", "answer_data": [ "authority", "method", "evidence", "event time", "knowledge time" ] }, { "id": "distinguishing-features-q03", "text": "What may an agent decide or do once what distinguishes a L-isoleucine is known, and what must it refuse?", "kind": "action", "answer_data": [ "permitted actions", "preconditions", "refusals", "escalation" ] } ] } ] } ] }, { "id": "form-and-composition", "name": "Form, composition and condition", "description": "What a L-isoleucine is made of, what shape and size it takes, and what state it is in.", "rationale": "A physical thing can be measured, and a model that cannot record its measurements cannot support any decision that depends on them.", "layers": [ { "id": "form-and-dimensions", "name": "Form and dimensions", "description": "Shape, size, mass and the ranges these take across examples.", "findings": [ { "id": "measurable-dimensions", "name": "Measurable dimensions and their units", "description": "The quantities worth recording and the units they are recorded in.", "questions": [ { "id": "measurable-dimensions-q01", "text": "What identifies and describes the dimensions of a L-isoleucine, and in what units or vocabulary?", "kind": "definition", "answer_data": [ "identifiers", "types and classes", "values with units", "explicit unknowns" ] }, { "id": "measurable-dimensions-q02", "text": "Who or what asserted this about the dimensions of a L-isoleucine, by which method, and when was it true?", "kind": "provenance", "answer_data": [ "authority", "method", "evidence", "event time", "knowledge time" ] }, { "id": "measurable-dimensions-q03", "text": "What may an agent decide or do once the dimensions of a L-isoleucine is known, and what must it refuse?", "kind": "action", "answer_data": [ "permitted actions", "preconditions", "refusals", "escalation" ] } ] } ] }, { "id": "material-and-composition", "name": "Material and composition", "description": "Materials, parts and how they are put together.", "findings": [ { "id": "parts-and-materials", "name": "Parts and materials", "description": "What a L-isoleucine is built from and which parts are replaceable.", "questions": [ { "id": "parts-and-materials-q01", "text": "What identifies and describes the composition of a L-isoleucine, and in what units or vocabulary?", "kind": "definition", "answer_data": [ "identifiers", "types and classes", "values with units", "explicit unknowns" ] }, { "id": "parts-and-materials-q02", "text": "Who or what asserted this about the composition of a L-isoleucine, by which method, and when was it true?", "kind": "provenance", "answer_data": [ "authority", "method", "evidence", "event time", "knowledge time" ] }, { "id": "parts-and-materials-q03", "text": "What may an agent decide or do once the composition of a L-isoleucine is known, and what must it refuse?", "kind": "action", "answer_data": [ "permitted actions", "preconditions", "refusals", "escalation" ] } ] } ] }, { "id": "condition-and-integrity", "name": "Condition and integrity", "description": "Wear, damage, health and the difference between working and broken.", "findings": [ { "id": "condition-assessment", "name": "Condition, damage and health", "description": "How the condition of a L-isoleucine is judged and by whom.", "questions": [ { "id": "condition-assessment-q01", "text": "What identifies and describes the condition of a L-isoleucine, and in what units or vocabulary?", "kind": "definition", "answer_data": [ "identifiers", "types and classes", "values with units", "explicit unknowns" ] }, { "id": "condition-assessment-q02", "text": "Who or what asserted this about the condition of a L-isoleucine, by which method, and when was it true?", "kind": "provenance", "answer_data": [ "authority", "method", "evidence", "event time", "knowledge time" ] }, { "id": "condition-assessment-q03", "text": "What may an agent decide or do once the condition of a L-isoleucine is known, and what must it refuse?", "kind": "action", "answer_data": [ "permitted actions", "preconditions", "refusals", "escalation" ] } ] } ] } ] }, { "id": "place-and-movement", "name": "Place and movement", "description": "Where a L-isoleucine is, how it got there and where it may go (varies).", "rationale": "A thing that can move needs its position recorded with a time, or every later claim about it is about a place it has left.", "layers": [ { "id": "location-and-placement", "name": "Location and placement", "description": "Position, containment and the reference frame the position is given in.", "findings": [ { "id": "position-and-frame", "name": "Position, container and reference frame", "description": "Where a L-isoleucine is, and what that position is measured against.", "questions": [ { "id": "position-and-frame-q01", "text": "What identifies and describes the location of a L-isoleucine, and in what units or vocabulary?", "kind": "definition", "answer_data": [ "identifiers", "types and classes", "values with units", "explicit unknowns" ] }, { "id": "position-and-frame-q02", "text": "Who or what asserted this about the location of a L-isoleucine, by which method, and when was it true?", "kind": "provenance", "answer_data": [ "authority", "method", "evidence", "event time", "knowledge time" ] }, { "id": "position-and-frame-q03", "text": "What may an agent decide or do once the location of a L-isoleucine is known, and what must it refuse?", "kind": "action", "answer_data": [ "permitted actions", "preconditions", "refusals", "escalation" ] } ] } ] }, { "id": "movement-and-transfer", "name": "Movement and transfer", "description": "How a L-isoleucine is carried, installed or relocated.", "findings": [ { "id": "movement-events", "name": "Movement events and constraints", "description": "What counts as a movement, what records it and what limits it.", "questions": [ { "id": "movement-events-q01", "text": "What identifies and describes the movement of a L-isoleucine, and in what units or vocabulary?", "kind": "definition", "answer_data": [ "identifiers", "types and classes", "values with units", "explicit unknowns" ] }, { "id": "movement-events-q02", "text": "Who or what asserted this about the movement of a L-isoleucine, by which method, and when was it true?", "kind": "provenance", "answer_data": [ "authority", "method", "evidence", "event time", "knowledge time" ] }, { "id": "movement-events-q03", "text": "What may an agent decide or do once the movement of a L-isoleucine is known, and what must it refuse?", "kind": "action", "answer_data": [ "permitted actions", "preconditions", "refusals", "escalation" ] } ] } ] } ] }, { "id": "state-and-lifecycle", "name": "State and lifecycle", "description": "The states a L-isoleucine passes through and the events that move it between them.", "rationale": "Most decisions about a thing depend on what state it is in now, which is a claim with a time on it, not a property.", "layers": [ { "id": "lifecycle-stages", "name": "Lifecycle stages", "description": "From coming into existence to ceasing to be one of these.", "findings": [ { "id": "stages-and-transitions", "name": "Stages and transitions", "description": "The stages worth naming and what moves a L-isoleucine between them.", "questions": [ { "id": "stages-and-transitions-q01", "text": "What identifies and describes the lifecycle of a L-isoleucine, and in what units or vocabulary?", "kind": "definition", "answer_data": [ "identifiers", "types and classes", "values with units", "explicit unknowns" ] }, { "id": "stages-and-transitions-q02", "text": "Who or what asserted this about the lifecycle of a L-isoleucine, by which method, and when was it true?", "kind": "provenance", "answer_data": [ "authority", "method", "evidence", "event time", "knowledge time" ] }, { "id": "stages-and-transitions-q03", "text": "What may an agent decide or do once the lifecycle of a L-isoleucine is known, and what must it refuse?", "kind": "action", "answer_data": [ "permitted actions", "preconditions", "refusals", "escalation" ] } ] } ] }, { "id": "observations-and-status", "name": "Observations and current status", "description": "What is observed about a L-isoleucine, how often and by whom.", "findings": [ { "id": "observation-record", "name": "Observation record", "description": "How an observation of a L-isoleucine is recorded so that it can be superseded rather than overwritten.", "questions": [ { "id": "observation-record-q01", "text": "What identifies and describes an observation of a L-isoleucine, and in what units or vocabulary?", "kind": "definition", "answer_data": [ "identifiers", "types and classes", "values with units", "explicit unknowns" ] }, { "id": "observation-record-q02", "text": "Who or what asserted this about an observation of a L-isoleucine, by which method, and when was it true?", "kind": "provenance", "answer_data": [ "authority", "method", "evidence", "event time", "knowledge time" ] }, { "id": "observation-record-q03", "text": "What may an agent decide or do once an observation of a L-isoleucine is known, and what must it refuse?", "kind": "action", "answer_data": [ "permitted actions", "preconditions", "refusals", "escalation" ] } ] } ] } ] }, { "id": "use-and-operation", "name": "Use and operation", "description": "What can be done with a L-isoleucine and what it takes to do it.", "rationale": "An agent that can recognise a thing but not act on it has only half a model.", "layers": [ { "id": "intended-use", "name": "Intended use and function", "description": "What a L-isoleucine is for, and what it is used for anyway.", "findings": [ { "id": "function-and-purpose", "name": "Function, purpose and misuse", "description": "The function a L-isoleucine performs and the uses that fall outside it.", "questions": [ { "id": "function-and-purpose-q01", "text": "What identifies and describes the use of a L-isoleucine, and in what units or vocabulary?", "kind": "definition", "answer_data": [ "identifiers", "types and classes", "values with units", "explicit unknowns" ] }, { "id": "function-and-purpose-q02", "text": "Who or what asserted this about the use of a L-isoleucine, by which method, and when was it true?", "kind": "provenance", "answer_data": [ "authority", "method", "evidence", "event time", "knowledge time" ] }, { "id": "function-and-purpose-q03", "text": "What may an agent decide or do once the use of a L-isoleucine is known, and what must it refuse?", "kind": "action", "answer_data": [ "permitted actions", "preconditions", "refusals", "escalation" ] } ] } ] }, { "id": "handling-and-procedure", "name": "Handling and procedure", "description": "The steps, skills and tools involved in using a L-isoleucine.", "findings": [ { "id": "procedures-and-skills", "name": "Procedures, skills and required tools", "description": "What an agent needs before it can act on a L-isoleucine at all.", "questions": [ { "id": "procedures-and-skills-q01", "text": "What identifies and describes handling a L-isoleucine, and in what units or vocabulary?", "kind": "definition", "answer_data": [ "identifiers", "types and classes", "values with units", "explicit unknowns" ] }, { "id": "procedures-and-skills-q02", "text": "Who or what asserted this about handling a L-isoleucine, by which method, and when was it true?", "kind": "provenance", "answer_data": [ "authority", "method", "evidence", "event time", "knowledge time" ] }, { "id": "procedures-and-skills-q03", "text": "What may an agent decide or do once handling a L-isoleucine is known, and what must it refuse?", "kind": "action", "answer_data": [ "permitted actions", "preconditions", "refusals", "escalation" ] } ] } ] }, { "id": "supply-and-consumption", "name": "Supply and consumption", "description": "Quantities, rates and what running out means.", "findings": [ { "id": "consumption-and-stock", "name": "Consumption, stock and replenishment", "description": "How much of a L-isoleucine there is and how fast it goes.", "questions": [ { "id": "consumption-and-stock-q01", "text": "What identifies and describes the supply of a L-isoleucine, and in what units or vocabulary?", "kind": "definition", "answer_data": [ "identifiers", "types and classes", "values with units", "explicit unknowns" ] }, { "id": "consumption-and-stock-q02", "text": "Who or what asserted this about the supply of a L-isoleucine, by which method, and when was it true?", "kind": "provenance", "answer_data": [ "authority", "method", "evidence", "event time", "knowledge time" ] }, { "id": "consumption-and-stock-q03", "text": "What may an agent decide or do once the supply of a L-isoleucine is known, and what must it refuse?", "kind": "action", "answer_data": [ "permitted actions", "preconditions", "refusals", "escalation" ] } ] } ] } ] }, { "id": "composition-and-identity", "name": "Composition, grade and chemical identity", "description": "What L-isoleucine is made of and how pure the thing in hand is.", "rationale": "Grade decides what a substance may be used for, so a model that records only the name cannot support any decision about use.", "layers": [ { "id": "chemical-identity", "name": "Chemical identity", "description": "Formula, structure and the registry numbers that name it unambiguously.", "findings": [ { "id": "identity-record", "name": "Identity and registry numbers", "description": "What identifies this substance across suppliers and regulators.", "questions": [ { "id": "identity-record-q01", "text": "What formula, structure and registry identifiers name L-isoleucine unambiguously?", "kind": "definition" }, { "id": "identity-record-q02", "text": "Which related substances share a name in common use but not an identifier?", "kind": "boundary" } ] } ] }, { "id": "grade-and-impurities", "name": "Grade, purity and impurities", "description": "The specification a batch meets and what else is in it.", "findings": [ { "id": "specification", "name": "Specification and assay", "description": "How purity is stated and measured, and which impurities matter.", "questions": [ { "id": "specification-q01", "text": "What grades of L-isoleucine exist, how is purity assayed, and which impurities are limited?", "kind": "measurement" }, { "id": "specification-q02", "text": "Which use requires which grade, and what happens when a lower grade is substituted?", "kind": "action" } ] } ] } ] }, { "id": "properties-and-behaviour", "name": "Properties and behaviour", "description": "How L-isoleucine behaves under conditions an agent can measure or change.", "rationale": "Physical constants are the part of a substance model that transfers between contexts, and each needs its unit and its conditions.", "layers": [ { "id": "physical-constants", "name": "Physical constants", "description": "Values that hold for the pure substance, with their conditions.", "findings": [ { "id": "constants-record", "name": "Constants and conditions", "description": "The quantities worth recording and the state they apply in.", "questions": [ { "id": "constants-record-q01", "text": "What are the defining physical constants of L-isoleucine, in what units and under what conditions?", "kind": "measurement" }, { "id": "constants-record-q02", "text": "Which of these values change with grade or form rather than being fixed?", "kind": "boundary" } ] } ] }, { "id": "stability-and-reactivity", "name": "Stability and reactivity", "description": "What it reacts with, degrades into, and how it is stored.", "findings": [ { "id": "incompatibilities", "name": "Incompatibilities and degradation", "description": "What must not meet it, and what it becomes over time.", "questions": [ { "id": "incompatibilities-q01", "text": "What is L-isoleucine incompatible with, and what does it degrade into under storage?", "kind": "definition" }, { "id": "incompatibilities-q02", "text": "What storage and handling does that require, and who is competent to do it?", "kind": "action" } ] } ] } ] }, { "id": "hazard-and-regulation", "name": "Hazard classification and regulation", "description": "How L-isoleucine can harm, and what the law says about holding and moving it.", "rationale": "A substance model without its hazard class and its regulatory identity is unusable in exactly the situations that matter.", "layers": [ { "id": "hazard-class", "name": "Hazard classification", "description": "Classified hazards, exposure limits and protective measures.", "findings": [ { "id": "classified-hazards", "name": "Classified hazards and limits", "description": "The classification, the scheme it belongs to, and the limits set.", "questions": [ { "id": "classified-hazards-q01", "text": "How is L-isoleucine classified for hazard, under which scheme, and what exposure limits apply?", "kind": "definition" }, { "id": "classified-hazards-q02", "text": "What protection does handling require, and what must an agent refuse to do without it?", "kind": "action" } ] } ] }, { "id": "regulatory-identity", "name": "Regulatory identity and control", "description": "Registration, restriction and transport rules by jurisdiction.", "findings": [ { "id": "controls", "name": "Controls and jurisdiction", "description": "Where it is restricted and what the restriction attaches to.", "questions": [ { "id": "controls-q01", "text": "Which registrations, restrictions or transport rules cover L-isoleucine, and where?", "kind": "provenance" }, { "id": "controls-q02", "text": "Does the control attach to the substance, its concentration, or its intended use?", "kind": "boundary" } ] } ] } ] }, { "id": "provenance-and-evidence", "name": "Provenance, evidence and time", "description": "Where every claim about a L-isoleucine came from and when it held.", "rationale": "A claim without a source and a time cannot be superseded, only overwritten, and an agent that overwrites loses the ability to explain itself.", "layers": [ { "id": "source-and-authority", "name": "Source and authority", "description": "Who said it, on what evidence, and how strongly.", "findings": [ { "id": "claim-provenance", "name": "Claim provenance and confidence", "description": "The authority behind each claim about a L-isoleucine and how confident it is.", "questions": [ { "id": "claim-provenance-q01", "text": "What identifies and describes a claim about a L-isoleucine, and in what units or vocabulary?", "kind": "definition", "answer_data": [ "identifiers", "types and classes", "values with units", "explicit unknowns" ] }, { "id": "claim-provenance-q02", "text": "Who or what asserted this about a claim about a L-isoleucine, by which method, and when was it true?", "kind": "provenance", "answer_data": [ "authority", "method", "evidence", "event time", "knowledge time" ] }, { "id": "claim-provenance-q03", "text": "What may an agent decide or do once a claim about a L-isoleucine is known, and what must it refuse?", "kind": "action", "answer_data": [ "permitted actions", "preconditions", "refusals", "escalation" ] } ] } ] }, { "id": "time-and-versions", "name": "Time, versions and supersession", "description": "When a claim was true, when it was learnt, and what replaced it.", "findings": [ { "id": "validity-and-supersession", "name": "Validity period and supersession", "description": "How an old claim about a L-isoleucine is retired without being erased.", "questions": [ { "id": "validity-and-supersession-q01", "text": "What identifies and describes the validity of a claim about a L-isoleucine, and in what units or vocabulary?", "kind": "definition", "answer_data": [ "identifiers", "types and classes", "values with units", "explicit unknowns" ] }, { "id": "validity-and-supersession-q02", "text": "Who or what asserted this about the validity of a claim about a L-isoleucine, by which method, and when was it true?", "kind": "provenance", "answer_data": [ "authority", "method", "evidence", "event time", "knowledge time" ] }, { "id": "validity-and-supersession-q03", "text": "What may an agent decide or do once the validity of a claim about a L-isoleucine is known, and what must it refuse?", "kind": "action", "answer_data": [ "permitted actions", "preconditions", "refusals", "escalation" ] } ] } ] } ] } ] }, "statistics": { "bundles": 9, "layers": 20, "findings": 22, "questions": 60 } } } }