leucine-rich repeat proteins

Registered family meaning

Record the intended breadth of the existing registry entry without creating an unsupported narrower family.

1. Does the originating registry record denote an LRR repeat class, an LRR-containing protein grouping or a specific homologous family? definition
2. Which existing models already cover this concept or particular member families? boundary

Supported LRR assignment

Require interpretable evidence for repeats and retain uncertainty around isolated or conflicting matches.

1. Which sequence accession, version and residue intervals were assessed? provenance
2. Which repeat profiles or structural observations support the assignment, and with what scores or confidence? measurement
3. Could the match reflect leucine enrichment, low complexity or a different repeat architecture? boundary

Repeat map

Maintain an evidence-backed repeat map rather than a count inferred solely from domain length.

1. How many repeats are supported, and what are their individual residue intervals and lengths? measurement
2. Where do sequence-based and structure-based repeat boundaries disagree? boundary

Insertions and divergent repeats

Record insertions, deletions and divergent units without automatically treating them as annotation errors.

1. Which repeat positions contain insertions, deletions or departures from the assigned repeat profile? measurement
2. Does each atypical segment remain part of the LRR array, form a separate domain or remain unresolved? boundary

Observed or predicted fold

Keep experimental structures and predictions distinguishable when assessing repeat packing and array conformation.

1. Which structure, chain and residue coverage support the LRR fold assignment? provenance
2. Which portions of the repeat array are experimentally resolved, confidently predicted or structurally uncertain? measurement

Cap preservation and folding

Treat cap assignments and folding requirements as member-specific evidence needed for construct interpretation.

1. What supports the proposed N-terminal and C-terminal caps or alternative protection of the array ends? provenance
2. Under the tested conditions, what measurements distinguish a folded LRR construct from an unfolded or aggregated preparation? measurement
3. Which proposed truncations require additional folding checks before their binding results can be interpreted? action

LRR context map

Relate the LRR region to neighbouring domains and its supported cellular setting.

1. Which domains, signal sequences or membrane-spanning segments flank the LRR region? boundary
2. What evidence establishes the cellular compartment and membrane orientation, where relevant? provenance

LRR-mediated binding

Separate direct binding and mapped interfaces from whole-protein association or downstream responses.

1. Which partner binds the tested LRR-containing construct, and what establishes a direct interaction? provenance
2. Which repeat residues or surfaces are implicated by structural or perturbation evidence? measurement
3. Can the observed association be assigned to the LRR region rather than a neighbouring domain or bridging molecule? boundary

Variant effect separation

Keep measured effects separate from predictions about repeat packing or partner recognition.

1. Does the variant alter a repeat core, cap, candidate binding surface or repeat count? boundary
2. What measurements separate altered affinity from altered abundance, localisation or folding? measurement
3. Which matched control would best distinguish these explanations? action

Evidence-limited next action

Choose follow-up actions that address specific uncertainties and define when an annotation can be strengthened.

1. Which construct boundaries preserve the supported LRR array and terminal protection? action
2. What additional evidence would justify confirming the repeat assignment or transferring a functional annotation to another member? action
3. Which conclusion remains limited to the tested isoform, construct, partner and assay conditions? boundary

Check these first

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these check these first hold for the sense of Leucine-rich repeat, protein family this model covers, and on what evidence? provenance

Kinds and varieties

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these kinds and varieties hold for the sense of Leucine-rich repeat, protein family this model covers, and on what evidence? provenance

Identifiers and schemes

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these identifiers and schemes hold for the sense of Leucine-rich repeat, protein family this model covers, and on what evidence? provenance

Real-world use

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these real-world use hold for the sense of Leucine-rich repeat, protein family this model covers, and on what evidence? provenance

Typical measurements

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these typical measurements hold for the sense of Leucine-rich repeat, protein family this model covers, and on what evidence? provenance

Failure modes and hazards

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these failure modes and hazards hold for the sense of Leucine-rich repeat, protein family this model covers, and on what evidence? provenance

Neighbouring kinds and how to tell them apart

Recalled without web access and unsourced; every item is a lead to verify.

1. Which of these neighbouring kinds and how to tell them apart hold for the sense of Leucine-rich repeat, protein family this model covers, and on what evidence? provenance