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Research draft

electrophoresis

vr.tr.electrophoresis · ACT.PRC

Let an agent explain electrophoresis by method, equipment, interpretation, safety and applications, without interpreting individual medical results.

Thing Registry Activities and processes

Research draft, second pass

A second pass drafted this model: the structure a model of this thing needs, and what is known about it in the world. The line under this one says how the second half was obtained - researched against sources, or recalled without web access, in which case nothing here was read anywhere and every claim is a lead to verify. Unreviewed either way.

written by Claude from model knowledge without web access - no source was read, every claim is a lead to verify

Researched by: Claude

Purpose and description

Let an agent explain electrophoresis by method, equipment, interpretation, safety and applications, without interpreting individual medical results.

A laboratory technique that separates charged molecules such as DNA, RNA and proteins by their movement through a gel, capillary or fluid under an electric field, according to size, charge and shape; used in research, forensics and clinical tests such as serum protein electrophoresis.

What it is for: Separating and analysing biomolecules.

It can be choose a type of electrophoresis; explain how gels are run and read; follow lab safety for stains and voltage; describe clinical and forensic uses in general terms.

Distinguishing features

Uses an electric field

Separates by size and charge

Needs markers for comparison

Widely used in molecular biology

What it looks like

Gel slabs in buffer tanks with bands, or capillary instruments producing electropherograms.

How it is recognised

Bands on agarose or polyacrylamide gels

Ladders of size markers

Chromatography separates by different principles

Related models

is a kind of - category

separation process

separates - analytes

nucleic acids and proteins

is used in - application

DNA profiling

is contrasted with - alternative

chromatography

In practice

Families and kinds

agarose gel electrophoresis

SDS-PAGE

capillary electrophoresis

two-dimensional electrophoresis

clinical protein electrophoresis

Standards and regulation

Laboratory electrical safety rules

Chemical safety for stains and acrylamide

Clinical laboratory accreditation

Failure modes and hazards

Electric shock from power supplies

Toxic stains and unpolymerised acrylamide

Misreading bands without controls

Also called

microchip electrophoresisGel electrophoresis of nucleic acidsserum protein electrophoresisFree-flow electrophoresiszone electrophoresislipoprotein electrophoresisisotachophoresiscrossover electrophoresisAffinity electrophoresisgel electrophoresispaper electrophoresiscellulose acetate electrophoresiscapillary electrophoresisimmunoelectrophoresisnative electrophoresisisoelectric focusingdielectrophoresisgel electrophoresis of proteinsZymographyDiscontinuous Electrophoresisgradient electrophoresisColorless Native Polyacrylamide Gel Electrophoresispolyacrylamide gel electrophoresistwo-dimensional gel electrophoresisDifference gel electrophoresisQPNC-PAGEstarch gel electrophoresistwo-dimensional difference gel electrophoresispulsed field gel electrophoresisagarose gel electrophoresiscapillary isoelectric focusingcounterimmunoelectrophoresistwo-dimensional immunoelectrophoresisnative polyacrylamide gel electrophoresisBAC-PAGECTAB-PAGEdisc electrophoresisdenaturing gradient gel electrophoresisSDD-AGE

Where this came from

wikidata · CC0 1.0

Source repository Registry entry point

Also registered as vr.tr.electrophoresis

Drafted structure

Bundle to layer to finding to question, as the second pass will find it: 4 bundles · 8 layers · 8 findings · 16 questions.

Method Technique.

Choice depends on sample.

Type

Which technique.

Type

Technique choice.

  1. Which electrophoresis technique suits this sample? definition
  2. What gel percentage or buffer is appropriate? measurement

Run

Running a gel.

Run

Running.

  1. What voltage and time are typical for this gel? measurement
  2. Which protocol source is used? provenance
Reading Interpretation.

Controls enable reading.

Bands

Sizes.

Bands

Band sizes.

  1. How are band sizes estimated against a ladder? measurement
  2. What could smears or extra bands mean? definition

Clinical

Medical tests.

Clinical

Clinical tests.

  1. What does serum protein electrophoresis test for, in general terms? provenance
  2. Is the user asking about their own result? boundary
Safety Hazards.

Voltage and chemicals.

Electrical

Shock.

Electrical

Electrical safety.

  1. Is the power supply off before lids are opened? boundary
  2. What are lab rules for high-voltage equipment? provenance

Chemical

Stains.

Chemical

Chemical safety.

  1. Which stains or reagents are hazardous, and what protection is needed? provenance
  2. How is waste disposed of? provenance
Applications Uses.

Uses are broad.

Research

Molecular biology.

Research

Research uses.

  1. How is electrophoresis used in cloning and PCR checks? definition
  2. Which alternatives exist? definition

Forensic

DNA profiling.

Forensic

Forensic uses.

  1. How is capillary electrophoresis used in DNA profiling, in general terms? definition
  2. Which standards govern forensic labs? provenance

What the second pass must settle

  • Should techniques be separate entries?
  • How should protocols be linked?
  • How should clinical tests be linked?
Draft as JSON